caa a22 4500 477135722 CHVBK 20180405111717.0 cr unu---uuuuu 170330e19970501xx s 000 0 eng 10.1023/A:1002910114869 doi (NATIONALLICENCE)springer-10.1023/A:1002910114869 Genetic diversity in Lima bean (Phaseolus lunatus L.) as revealed by RAPD markers [Elektronische Daten] [B. Fofana, X. Vekemans, P. du Jardin, J.P. Baudoin] The genetic variability of 46 accessions of the Lima bean (P. lunatus L.) including 16 wild forms and 30 landraces belonging to the three cultigroups Big lima, Sieva, Potato, and their intermediates, was evaluated using RAPD (Random amplified polymorphic DNA) markers. Twelve oligonucleotide primers produced 172 RAPD markers which allowed the differentiation of two main groups: the mesoamerican and the andean groups. This was confirmed by an AMOVA analysis which indicated that 37.7% of the variation was found between these two groups. For each botanical form (wild and cultivated), the molecular markers showed that small-seeded types (i.e. Sieva and Potato types and their related wild forms) had a wide distribution (from Mexico to Argentina) while the large-seeded types (Big lima type and its related wild forms) were circumscribed to the narrow west-coastal region from Ecuador to Bolivia. The results are in favour of an independent domestication process within the two groups, as the differentiation between mesoamerican and andean accessions was found to occur in both wild forms and landraces. Within each of the two main groups, wild forms and landraces were also found to be genetically differentiated and higher genetic diversity was observed among landraces than among wild forms. Within the mesoamerican landraces, low but significant differentiation between the Sieva and Potato cultigroups was observed. Some suggestions and hypotheses are discussed about evolution of the two small-seeded types. Kluwer Academic Publishers, 1997 andean nationallicence cultigroups nationallicence genetic variability nationallicence Lima bean nationallicence mesoamerican nationallicence RAPD nationallicence Fofana B. Unité de Biologie végétale, Belgium aut Vekemans X. Laboratoire de Génétique et d'Ecologie végétales, Université Libre de Bruxelles, 1850 Chaussée de Wavre, B-1160, Brussels, Belgium aut du Jardin P. Unité de Biologie végétale, Belgium aut Baudoin J.P. Faculté universitaire des Sciences agronomiques de Gembloux, Unité de Phytotechnie des Régions intertropicales, 2 Passage des Déportés, B-5030, Gembloux, Belgium aut Euphytica Kluwer Academic Publishers 95/2(1997-05-01), 157-165 0014-2336 95:2<157 1997 95 10681 https://doi.org/10.1023/A:1002910114869 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1002910114869 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fofana B. Unité de Biologie végétale, Belgium aut NATIONALLICENCE 700 1- Vekemans X. Laboratoire de Génétique et d'Ecologie végétales, Université Libre de Bruxelles, 1850 Chaussée de Wavre, B-1160, Brussels, Belgium aut NATIONALLICENCE 700 1- du Jardin P. Unité de Biologie végétale, Belgium aut NATIONALLICENCE 700 1- Baudoin J.P. Faculté universitaire des Sciences agronomiques de Gembloux, Unité de Phytotechnie des Régions intertropicales, 2 Passage des Déportés, B-5030, Gembloux, Belgium aut NATIONALLICENCE 773 0- Euphytica Kluwer Academic Publishers 95/2(1997-05-01), 157-165 0014-2336 95:2<157 1997 95 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer