caa a22 4500 47713582X CHVBK 20180405111717.0 cr unu---uuuuu 170330e19970701xx s 000 0 eng 10.1023/A:1018331001961 doi (NATIONALLICENCE)springer-10.1023/A:1018331001961 Integration of spin-state-selective excitation into 2D NMR correlation experiments with heteronuclear ZQ/2Q π rotations for1 JXH [Elektronische Daten] [Axel Meissner, Jens Duus, Ole Sørensen] Spin-State-Selective Excitation (S3E), which forexample selectively excites amide proton resonances corresponding toexclusively either the α or the β spin state of the covalentlybound 15N atom is employed for E.COSY-type extraction ofheteronuclear J coupling constants. Instead of having one spectrum with twopeaks (corresponding to the α or β spin state of15N), S3E generates two spectra, each with onlyone peak for each 15N nucleus. These two spectra are generatedfrom the same data set, so that there is no reduction in sensitivitycompared to conventional 1JNH-resolved methods.Another interesting feature in comparison with conventional methods is that1JNH can be suppressed during the evolutionperiod, meaning that no heteronuclear multiplet structure is visible in theω1 frequency dimension. The S3E pulsesequence element is combined with NOESY for measurement of3JN-Hβ and JN-Hαcoupling constants in either a hetero- or a homonuclear correlated version.Experimental confirmation is obtained using the protein RAP 17-;97(N-terminal domain of α2-macroglobulin ReceptorAssociated Protein). Kluwer Academic Publishers, 1997 E.COSY nationallicence Multidimensional NMR nationallicence J coupling constants nationallicence S3E nationallicence Meissner Axel Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut Duus Jens Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut Sørensen Ole Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/1(1997-07-01), 89-94 0925-2738 10:1<89 1997 10 10858 https://doi.org/10.1023/A:1018331001961 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018331001961 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meissner Axel Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut NATIONALLICENCE 700 1- Duus Jens Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut NATIONALLICENCE 700 1- Sørensen Ole Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/1(1997-07-01), 89-94 0925-2738 10:1<89 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer