caa a22 4500 477135846 CHVBK 20180405111717.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1018301818803 doi (NATIONALLICENCE)springer-10.1023/A:1018301818803 Specific 15N, NH correlations for residues in15 N, 13C and fractionally deuterated proteins that immediately follow methyl-containing amino acids [Elektronische Daten] [D.R. Muhandiram, Philip Johnson, Daiwen Yang, Ouwen Zhang, Lawrence McIntosh, Lewis Kay] A triple-resonance pulse scheme is described which records15N, NH correlations of residues that immediately follow amethyl-containing amino acid. The experiment makes use of a15N, 13C and fractionally deuterated proteinsample and selects for CH2D methyl types. The experiment isthus useful in the early stages of the sequential assignment process as wellas for the confirmation of backbone 15N, NH chemical shiftassignments at later stages of data analysis. A simple modification of thesequence also allows the measurement of methyl side-chain dynamics. This isparticularly useful for studying side-chain dynamic properties in partiallyunfolded and unfolded proteins where the resolution of aliphatic carbon andproton chemical shifts is limited compared to that of amide nitrogens. Kluwer Academic Publishers, 1997 15N,13C,2H-labeled proteins nationallicence 2H spin relaxation nationallicence 15N, NH correlations nationallicence Muhandiram D.R. The Protein Engineering Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S 1A8, ON, Canada aut Johnson Philip The Protein Engineering Centers of Excellence, Department of Chemistry and Department of Biochemistry and Molecular Biology, University of British Columbia, V6T 1Z3, Vancouver, BC, Canada aut Yang Daiwen The Protein Engineering Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S 1A8, ON, Canada aut Zhang Ouwen The Protein Engineering Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S 1A8, ON, Canada aut McIntosh Lawrence The Protein Engineering Centers of Excellence, Department of Chemistry and Department of Biochemistry and Molecular Biology, University of British Columbia, V6T 1Z3, Vancouver, BC, Canada aut Kay Lewis The Protein Engineering Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S 1A8, ON, Canada aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 283-288 0925-2738 10:3<283 1997 10 10858 https://doi.org/10.1023/A:1018301818803 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018301818803 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muhandiram D.R. The Protein Engineering Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S 1A8, ON, Canada aut NATIONALLICENCE 700 1- Johnson Philip The Protein Engineering Centers of Excellence, Department of Chemistry and Department of Biochemistry and Molecular Biology, University of British Columbia, V6T 1Z3, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Yang Daiwen The Protein Engineering Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S 1A8, ON, Canada aut NATIONALLICENCE 700 1- Zhang Ouwen The Protein Engineering Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S 1A8, ON, Canada aut NATIONALLICENCE 700 1- McIntosh Lawrence The Protein Engineering Centers of Excellence, Department of Chemistry and Department of Biochemistry and Molecular Biology, University of British Columbia, V6T 1Z3, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Kay Lewis The Protein Engineering Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, M5S 1A8, ON, Canada aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 283-288 0925-2738 10:3<283 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer