caa a22 4500 477135862 CHVBK 20180405111717.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1018358101811 doi (NATIONALLICENCE)springer-10.1023/A:1018358101811 Tritium NMR studies of the human carbonic anhydrase I-benzenesulfonamide complex [Elektronische Daten] [A.S. Culf, J.T. Gerig, P.G. Williams] Tritium NMR spectroscopy has been used to examine the complexformed by [4-3H]benzenesulfon-amide and human carbonicanhydrase I. The results show that in solution the inhibitor forms a 1:1complex with the enzyme. A 100-spin computational model of the system,constructed with reference to crystallographic results, was used tointerpret tritium relaxation behavior and 3H{1H}NOEs. The analysis shows that the rate of dissociation of theenzyme-sulfonamide complex is 0.35 s−1 and thatthe aromatic ring of the inhibitor undergoes rapid rotation while complexed. Kluwer Academic Publishers, 1997 Tritium nationallicence Enzyme nationallicence Carbonic anhydrase nationallicence Enzyme inhibitor nationallicence Aromatic ring rotation nationallicence Culf A.S. Department of Chemistry, University of California, 93106, Santa Barbara, CA, U.S.A aut Gerig J.T. Department of Chemistry, University of California, 93106, Santa Barbara, CA, U.S.A aut Williams P.G. The National Tritium Labelling Facility and Structural Biology Division, E.O. Lawrence Berkeley National Laboratory, One Cyclotron Road, 94720, Berkeley, CA, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 293-299 0925-2738 10:3<293 1997 10 10858 https://doi.org/10.1023/A:1018358101811 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018358101811 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Culf A.S. Department of Chemistry, University of California, 93106, Santa Barbara, CA, U.S.A aut NATIONALLICENCE 700 1- Gerig J.T. Department of Chemistry, University of California, 93106, Santa Barbara, CA, U.S.A aut NATIONALLICENCE 700 1- Williams P.G. The National Tritium Labelling Facility and Structural Biology Division, E.O. Lawrence Berkeley National Laboratory, One Cyclotron Road, 94720, Berkeley, CA, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 293-299 0925-2738 10:3<293 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer