caa a22 4500 477135900 CHVBK 20180405111717.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1018359305544 doi (NATIONALLICENCE)springer-10.1023/A:1018359305544 The button test: A small scale method using microdialysis cells for assessing protein solubility at concentrations suitable for NMR [Elektronische Daten] [Stefan Bagby, Kit Tong, Dingjiang Liu, Jean-René Alattia, Mitsuhiko Ikura] A simple method has been developed for screening solutionconditions to determine conditions under which a protein is soluble at thehigh concentrations typically used for NMR spectroscopy. The method employsmicrodialysis cells or ‘buttons'. The low sample volume (5 µl)required for each microdialysis button permits testing of a wide range ofsolution conditions and temperatures with high protein concentrations, usinga small amount of protein. Following precipitation of several NMR samples ofthe C-terminal core domain of human TFIIB, the microdialysis button screenfacilitated identification of conditions in which precipitation of the TFIIBcore domain was eliminated. The microdialysis button method for screeningsolution conditions is generally applicable and has been used to permitrapid identification of suitable NMR sample solution conditions for proteinsinvolved in transcription and cell adhesion. Kluwer Academic Publishers, 1997 Microdialysis cell nationallicence NMR sample preparation nationallicence Protein solubility nationallicence Solution condition screen nationallicence TFIIB nationallicence Bagby Stefan Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut Tong Kit Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut Liu Dingjiang Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut Alattia Jean-René Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut Ikura Mitsuhiko Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 279-282 0925-2738 10:3<279 1997 10 10858 https://doi.org/10.1023/A:1018359305544 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018359305544 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bagby Stefan Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut NATIONALLICENCE 700 1- Tong Kit Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut NATIONALLICENCE 700 1- Liu Dingjiang Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut NATIONALLICENCE 700 1- Alattia Jean-René Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut NATIONALLICENCE 700 1- Ikura Mitsuhiko Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9 aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 279-282 0925-2738 10:3<279 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer