caa a22 4500 477135927 CHVBK 20180405111717.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1018386217930 doi (NATIONALLICENCE)springer-10.1023/A:1018386217930 Three-dimensional structure of the Hck SH2 domain in solution [Elektronische Daten] [Weixing Zhang, Thomas Smithgall, William Gmeiner] The hematopoietic cellular kinase (Hck) is a member of the Srcfamily of non-receptor protein-tyrosine kinases that is expressedpredominantly in granulocytes, monocytes and macrophages. Recentobservations suggest that Hck may be activated in HIV-infected macrophagesand in chronic myelogenous leukemia cells that express Bcr-Abl. In order toincrease our understanding of the structural basis for regulation of Hckactivity under normal and pathological conditions, we have solved thesolution structure of the uncomplexed Hck SH2 domain using NMR spectroscopy.A novel procedure that uses intraresidueHN™Hα distances as references forconverting NOE intensities into distance restraints has been described. Atotal of 1757 significant experimental restraints were derived from NMRspectroscopic data including 238 medium-range and 487 long-range distancerestraints and 177 torsion angle restraints. These restraints were used in asimulated annealing procedure to generate 20 structures with the programDYANA. Superimposition of residues 5-104 upon the mean coordinate setyielded an average atomic rmsd values of 0.42 ± 0.08 Å for theN,Cα,C′ atoms and 0.81 ± 0.08 Å forall heavy atoms. Rmsd values for those residues in the regions of orderedsecondary structure were 0.27 ± 0.04 Å for theN,Cα,C′ atoms and 0.73 ± 0.06 Å forall heavy atoms. Kluwer Academic Publishers, 1997 Signal transduction nationallicence NOE reference nationallicence Improved pulse sequence nationallicence J-coupling nationallicence Stereospecific resonance assignment nationallicence Zhang Weixing The Eppley Institute for Research in Cancer and Allied Diseases, The University of Nebraska Medical Center, 68198-6805, Omaha, NE, U.S.A aut Smithgall Thomas The Eppley Institute for Research in Cancer and Allied Diseases, The University of Nebraska Medical Center, 68198-6805, Omaha, NE, U.S.A aut Gmeiner William The Eppley Institute for Research in Cancer and Allied Diseases, The University of Nebraska Medical Center, 68198-6805, Omaha, NE, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 263-272 0925-2738 10:3<263 1997 10 10858 https://doi.org/10.1023/A:1018386217930 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018386217930 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Weixing The Eppley Institute for Research in Cancer and Allied Diseases, The University of Nebraska Medical Center, 68198-6805, Omaha, NE, U.S.A aut NATIONALLICENCE 700 1- Smithgall Thomas The Eppley Institute for Research in Cancer and Allied Diseases, The University of Nebraska Medical Center, 68198-6805, Omaha, NE, U.S.A aut NATIONALLICENCE 700 1- Gmeiner William The Eppley Institute for Research in Cancer and Allied Diseases, The University of Nebraska Medical Center, 68198-6805, Omaha, NE, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 263-272 0925-2738 10:3<263 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer