caa a22 4500 477135935 CHVBK 20180405111717.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1018340506337 doi (NATIONALLICENCE)springer-10.1023/A:1018340506337 Conformation of an Shc-derived phosphotyrosine-containing peptide complexed with the Grb2 SH2 domain [Elektronische Daten] [Kenji Ogura, Shigeo Tsuchiya, Hiroaki Terasawa, Satoru Yuzawa, Hideki Hatanaka, Valsan Mandiyan, Joseph Schlessinger, Fuyuhiko Inagaki] We have determined the structure of an Shc-derivedphosphotyrosine-containing peptide complexed with Grb2 SH2 based on intra-and intermolecular NOE correlations observed by a series of isotope-filteredNMR experiments using a PFG z-filter. In contrast to an extendedconformation of phosphotyrosine-containing peptides bound to Src, Syp andPLC γ SH2s, the Shc-derived peptide formed a turn at the +1 and +2positions next to the phosphotyrosine residue. Trp121, locatedat the EF1 site of Grb2 SH2, blocked the peptide binding in an extendedconformation. The present study confirms that eachphosphotyrosine-containing peptide binds to the cognate SH2 with a specificconformation, which gives the structural basis for the binding specificitybetween SH2s and target proteins. Kluwer Academic Publishers, 1997 Grb2 nationallicence SH2 nationallicence Shc-derived peptide nationallicence Isotope-filtered NMR nationallicence Ogura Kenji Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut Tsuchiya Shigeo Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut Terasawa Hiroaki Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut Yuzawa Satoru Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut Hatanaka Hideki Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut Mandiyan Valsan Department of Pharmacology, New York University Medical Center, 10016, New York, NY, U.S.A aut Schlessinger Joseph Department of Pharmacology, New York University Medical Center, 10016, New York, NY, U.S.A aut Inagaki Fuyuhiko Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 273-278 0925-2738 10:3<273 1997 10 10858 https://doi.org/10.1023/A:1018340506337 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018340506337 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ogura Kenji Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Tsuchiya Shigeo Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Terasawa Hiroaki Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Yuzawa Satoru Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Hatanaka Hideki Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Mandiyan Valsan Department of Pharmacology, New York University Medical Center, 10016, New York, NY, U.S.A aut NATIONALLICENCE 700 1- Schlessinger Joseph Department of Pharmacology, New York University Medical Center, 10016, New York, NY, U.S.A aut NATIONALLICENCE 700 1- Inagaki Fuyuhiko Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 273-278 0925-2738 10:3<273 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer