caa a22 4500 47713596X CHVBK 20180405111718.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1018308717741 doi (NATIONALLICENCE)springer-10.1023/A:1018308717741 High-resolution heteronuclear NMR of human ubiquitin in an aqueous liquid crystalline medium [Elektronische Daten] [Ad Bax, Nico Tjandra] A mixture of dihexanoyl phosphatidylcholine and dimyristoylphosphatidylcholine in water forms disc-shaped particles, often referred toas bicelles [Sanders and Schwonek (1992) Biochemistry, 31, 8898-8905].These adopt an ordered, liquid crystalline phase, which can be maintained atvery low concentrations of the bicelles (down to 3% w/v). At thisconcentration the spacing between individual bicelles, on average, exceeds300 Å. The bicelles are shown to have a negligible effect on therotational diffusion of ubiquitin as judged by the 15NT1ρ values of the backbone amides relative to those inisotropic aqueous solution. The protein exhibits a residual degree ofalignment which is proportional to the bicelle concentration, andapproximately collinear with ubiquitin's rotational diffusion tensor. Thedegree of alignment obtained offers unique opportunities for studying theprotein's structure and dynamics. Kluwer Academic Publishers, 1997 Alignment nationallicence Bicelle nationallicence Dipolar coupling nationallicence Lipid nationallicence Liquid crystal nationallicence 15N relaxation nationallicence Bax Ad Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, 20892-0520, Bethesda, MD, U.S.A aut Tjandra Nico Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, 2089, Bethesda, MD aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 289-292 0925-2738 10:3<289 1997 10 10858 https://doi.org/10.1023/A:1018308717741 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018308717741 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bax Ad Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, 20892-0520, Bethesda, MD, U.S.A aut NATIONALLICENCE 700 1- Tjandra Nico Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, 2089, Bethesda, MD aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 289-292 0925-2738 10:3<289 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer