caa a22 4500 477135978 CHVBK 20180405111718.0 cr unu---uuuuu 170330e19971001xx s 000 0 eng 10.1023/A:1018358602001 doi (NATIONALLICENCE)springer-10.1023/A:1018358602001 Efficient enzymatic synthesis of 13 C,15N-labeled DNA for NMR studies [Elektronische Daten] [Deborah Smith, Jin-Yuan Su, Fiona Jucker] The power of heteronuclear NMR spectroscopy to study macromoleculesand their complexes has been amply demonstrated over the last decade. Theobstacle to routinely applying these techniques to the study of DNA has beenthe synthesis of 13C,15N-labeled DNA. Here wepresent a simple and efficient method to generate isotope-labeled DNA forNMR studies that is as easy as that for isotope labeling of RNA. The methodwas used to synthesize a uniformly13 C,15N-labeled 32-nucleotide DNA that binds tohuman basic fibroblast growth factor with high affinity and specificity.Isotope-edited experiments were applied to the13 C,15N-labeled DNA bound to unlabeled protein,and the 13 C,15N-labeled DNA was also examined incomplex with 15N-labeled protein. The NMR experiments showthat the DNA adopts a well-defined stable structure when bound to theprotein, and illustrate the potential of13 C,15N-labeled DNA for structural studies ofDNA-protein complexes. Kluwer Academic Publishers, 1997 DNA nationallicence DNA-protein complex nationallicence Deoxythymidylate kinase nationallicence Enzymatic synthesis nationallicence Heteronuclear NMR nationallicence Isotope labeling nationallicence Smith Deborah NeXstar Pharmaceuticals Inc., 2860 Wilderness Place, 80301, Boulder, CO, U.S.A aut Su Jin-Yuan NeXstar Pharmaceuticals Inc., 2860 Wilderness Place, 80301, Boulder, CO, U.S.A aut Jucker Fiona NeXstar Pharmaceuticals Inc., 2860 Wilderness Place, 80301, Boulder, CO, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 245-253 0925-2738 10:3<245 1997 10 10858 https://doi.org/10.1023/A:1018358602001 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018358602001 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith Deborah NeXstar Pharmaceuticals Inc., 2860 Wilderness Place, 80301, Boulder, CO, U.S.A aut NATIONALLICENCE 700 1- Su Jin-Yuan NeXstar Pharmaceuticals Inc., 2860 Wilderness Place, 80301, Boulder, CO, U.S.A aut NATIONALLICENCE 700 1- Jucker Fiona NeXstar Pharmaceuticals Inc., 2860 Wilderness Place, 80301, Boulder, CO, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/3(1997-10-01), 245-253 0925-2738 10:3<245 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer