caa a22 4500 477136028 CHVBK 20180405111718.0 cr unu---uuuuu 170330e19971201xx s 000 0 eng 10.1023/A:1018332327565 doi (NATIONALLICENCE)springer-10.1023/A:1018332327565 Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination [Elektronische Daten] [James Baleja, Venkataraman Thanabal, Gerhard Wagner] We have refined the solution structure of cadmium-bound GAL4 and present its15 N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 ± 0.05 Å for backbone atoms, and 0.9 ± 0.1 Å for all heavy atoms. The three-bond heteronuclear3 J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to3 J(113Cd,1H) are complex. Kluwer Academic Publishers, 1997 GAL4 nationallicence Cadmium nationallicence Coupling constant nationallicence Karplus relationship nationallicence Baleja James Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 02115, Boston, MA, U.S.A aut Thanabal Venkataraman Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 02115, Boston, MA, U.S.A aut Wagner Gerhard Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 02115, Boston, MA, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/4(1997-12-01), 397-401 0925-2738 10:4<397 1997 10 10858 https://doi.org/10.1023/A:1018332327565 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018332327565 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baleja James Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 02115, Boston, MA, U.S.A aut NATIONALLICENCE 700 1- Thanabal Venkataraman Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 02115, Boston, MA, U.S.A aut NATIONALLICENCE 700 1- Wagner Gerhard Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 02115, Boston, MA, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/4(1997-12-01), 397-401 0925-2738 10:4<397 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer