caa a22 4500 477136176 CHVBK 20180405111718.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1018323913680 doi (NATIONALLICENCE)springer-10.1023/A:1018323913680 Spin-state-selective coherence transfer via intermediate states of two-spin coherence in IS spin systems: Application to E.COSY-type measurement of J coupling constants [Elektronische Daten] [Morten Sørensen, Axel Meissner, Ole Sørensen] It is demonstrated that a new pulse sequence element, Spin-State-SelectiveCoherence Transfer (S3CT), via an intermediate state ofheteronuclear IS zero- or double-quantum coherence can transfer the twosingle-quantum coherences on one of the spins exclusively to any one of thetwo single-quantum coherences on the other spin. This fact is used for editinginto two subspectra that are most suitable for extraction of homo- orheteronuclear J coupling constants when S3CT is combined withhomonuclear coherence transfer during a mixing period. Experimentalconfirmation is obtained using a 15N-labeled 58-residue protein,the C-terminal Kunitz domain from human type VI collagen. The J coupling con-stants determined include 3JHN-Hα and3JN-Hβ related to the φ andχ1 angles, respectively. Kluwer Academic Publishers, 1997 E.COSY nationallicence Multidimensional NMR nationallicence J coupling constants nationallicence S3CT nationallicence Sørensen Morten Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut Meissner Axel Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut Sørensen Ole Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/2(1997-09-01), 181-186 0925-2738 10:2<181 1997 10 10858 https://doi.org/10.1023/A:1018323913680 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018323913680 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sørensen Morten Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut NATIONALLICENCE 700 1- Meissner Axel Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut NATIONALLICENCE 700 1- Sørensen Ole Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500, Valby, Denmark aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/2(1997-09-01), 181-186 0925-2738 10:2<181 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer