caa a22 4500 477136184 CHVBK 20180405111718.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1018381109524 doi (NATIONALLICENCE)springer-10.1023/A:1018381109524 Arginine side chain assignments in uniformly 15N-labeled proteins using the novel 2D HE(NE)HGHH experiment [Elektronische Daten] [Maurizio Pellecchia, Gerhard Wider, Hideo Iwai, Kurt Wüthrich] A novel 2D NMR experiment, 2D HE(NE)HGHH, is presented for the assignment ofarginine side chain 1H and 15N resonances inuniformly 15N-labeled proteins. Correlations between1Hε, 1Hγand 1Hη are established on the basis of3J(15N,1H) heteronuclear scalarcoupling constants, and sequence-specific assignments are obtained by overlapof these fragments with 1Hγ chemical shiftsobtained by assignment procedures starting from the polypeptide backbone.Since guanidino protons exchange quite rapidly with the bulk water, the 2DHE(NE)HGHH pulse scheme has been optimized to avoid saturation and dephasingof the water magnetization during the course of the experiment. As anillustration, arginine side chain assignments are presented for two uniformly15N-labeled proteins of 7 and 23 kDa molecular weight. Kluwer Academic Publishers, 1997 NMR assignments of arginine nationallicence Protein structure determination nationallicence Protein-DNA recognition nationallicence Pellecchia Maurizio Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland aut Wider Gerhard Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland aut Iwai Hideo Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland aut Wüthrich Kurt Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/2(1997-09-01), 193-197 0925-2738 10:2<193 1997 10 10858 https://doi.org/10.1023/A:1018381109524 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018381109524 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pellecchia Maurizio Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland aut NATIONALLICENCE 700 1- Wider Gerhard Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland aut NATIONALLICENCE 700 1- Iwai Hideo Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland aut NATIONALLICENCE 700 1- Wüthrich Kurt Eidgenössische Technische Hochschule-Hönggerberg, Institut für Molekularbiologie und Biophysik, CH-8093, Zürich, Switzerland aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/2(1997-09-01), 193-197 0925-2738 10:2<193 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer