caa a22 4500 477136192 CHVBK 20180405111718.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1018300215322 doi (NATIONALLICENCE)springer-10.1023/A:1018300215322 Towards structural investigations on isotope labelled native bacteriorhodopsin in detergent micelles by solution-state NMR spectroscopy [Elektronische Daten] [Heiko Patzelt, Anne Ulrich, Hermann Egbringhoff, Petra Düx, Jennifer Ashurst, Bernd Simon, Hartmut Oschkinat, Dieter Oesterhelt] 1H NMR signals of the retinal moiety in detergent-solubilizedbacteriorhodopsin are assigned, enabling the interpretation of NOEs within thechromophore. To achieve this, a number of differently labelled samples wereprepared to test the applicability of the various assignment and distancemeasurement strategies. In measurements with and without light,1H and 13C chemical shifts of the retinal in thenative protein were partially assigned for both the dark- and thelight-adapted states. Additionally, samples with residue-specific1H amino acids and/or retinal in an otherwise deuterated proteinwere prepared to measure the distances between either two kinds of amino acidsor between individual amino acids and the retinal moiety. With the observationof NOE within the bound retinal and between retinal and its neighbouring aminoacids, an important step towards the elucidation of distance constraints inthe binding pocket of the proton pump is made. Kluwer Academic Publishers, 1997 Bacteriorhodopsin nationallicence Structure nationallicence Membrane proteins nationallicence Isotope labelling nationallicence Patzelt Heiko Max-Planck-Institut für Biochemie, Am Klopferspitz, D-82152, Martinsried, Germany aut Ulrich Anne European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut Egbringhoff Hermann Max-Planck-Institut für Biochemie, Am Klopferspitz, D-82152, Martinsried, Germany aut Düx Petra European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut Ashurst Jennifer European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut Simon Bernd European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut Oschkinat Hartmut European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut Oesterhelt Dieter Max-Planck-Institut für Biochemie, Am Klopferspitz, D-82152, Martinsried, Germany aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/2(1997-09-01), 95-106 0925-2738 10:2<95 1997 10 10858 https://doi.org/10.1023/A:1018300215322 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018300215322 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Patzelt Heiko Max-Planck-Institut für Biochemie, Am Klopferspitz, D-82152, Martinsried, Germany aut NATIONALLICENCE 700 1- Ulrich Anne European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut NATIONALLICENCE 700 1- Egbringhoff Hermann Max-Planck-Institut für Biochemie, Am Klopferspitz, D-82152, Martinsried, Germany aut NATIONALLICENCE 700 1- Düx Petra European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut NATIONALLICENCE 700 1- Ashurst Jennifer European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut NATIONALLICENCE 700 1- Simon Bernd European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut NATIONALLICENCE 700 1- Oschkinat Hartmut European Molecular Biology Laboratory, P.O. Box 10 2209, D-69012, Heidelberg, Germany aut NATIONALLICENCE 700 1- Oesterhelt Dieter Max-Planck-Institut für Biochemie, Am Klopferspitz, D-82152, Martinsried, Germany aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/2(1997-09-01), 95-106 0925-2738 10:2<95 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer