caa a22 4500 477136214 CHVBK 20180405111718.0 cr unu---uuuuu 170330e19970901xx s 000 0 eng 10.1023/A:1018304117895 doi (NATIONALLICENCE)springer-10.1023/A:1018304117895 Characterisation of protein unfolding by NMR diffusion measurements [Elektronische Daten] [Jonathan Jones, Deborah Wilkins, Lorna Smith, Christopher Dobson] The characterisation of non-native states of proteins is a key problem instudies of protein folding. Complete characterisation of these states requiresa description of both local and global properties, including moleculardimensions. Here we present results from pulsed field gradient experimentsdesigned to compare the effective hydrodynamic radii of a protein in nativeand non-native states. Measurements performed on lysozyme indicate that theeffective hydrodynamic radius increases by 38±1% on unfolding in urea,a result completely consistent with a recent study by small-angle X-rayscattering. Kluwer Academic Publishers, 1997 Protein folding nationallicence Non-native states nationallicence Hydrodynamic radius nationallicence Lysozyme nationallicence Jones Jonathan Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QT, Oxford, U.K aut Wilkins Deborah Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QT, Oxford, U.K aut Smith Lorna Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QT, Oxford, U.K aut Dobson Christopher Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QT, Oxford, U.K aut Journal of Biomolecular NMR Kluwer Academic Publishers 10/2(1997-09-01), 199-203 0925-2738 10:2<199 1997 10 10858 https://doi.org/10.1023/A:1018304117895 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018304117895 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones Jonathan Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QT, Oxford, U.K aut NATIONALLICENCE 700 1- Wilkins Deborah Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QT, Oxford, U.K aut NATIONALLICENCE 700 1- Smith Lorna Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QT, Oxford, U.K aut NATIONALLICENCE 700 1- Dobson Christopher Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, OX1 3QT, Oxford, U.K aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 10/2(1997-09-01), 199-203 0925-2738 10:2<199 1997 10 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer