caa a22 4500 477136222 CHVBK 20180405111718.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1018679819693 doi (NATIONALLICENCE)springer-10.1023/A:1018679819693 (H)N(COCA)NH and HN(COCA)NH experiments for 1H-15N backbone assignments in 13C/15N-labeled proteins [Elektronische Daten] [Clay Bracken, Arthur III, John Cavanagh] Triple resonance HN(COCA)NH pulse sequences for correlating 1H(i), 15N(i),1H(i-1), and 15N(i-1) spins that utilize overlapping coherence transfer periods provide increased sensitivityrelative to pulse sequences that utilize sequential coherence transfer periods. Although theoverlapping sequence elements reduce the overall duration of the pulse sequences, theprincipal benefit derives from a reduction in the number of 180° pulses. Two versions of thetechnique are presented: a 3D (H)N(COCA)NH experiment that correlates 15N(i),1H(i-1), and 15N(i-1) spins, and a 3D HN(COCA)NH experiment that correlates 1H(i), 15N(i),1H(i-1), and 15N(i-1) spins by simultaneously encoding the 1H(i) and 15N(i) chemical shiftsduring the t1 evolution period. The methods are demonstrated on a 13C/15N-enriched sampleof the protein ubiquitin and are easily adapted for application to 2H/13C/15N-enrichedproteins. Kluwer Academic Publishers, 1997 Triple resonance NMR spectroscopy nationallicence Sequential assignment nationallicence Proteins nationallicence Bracken Clay Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, 10032, New York, NY, U.S.A aut III Arthur Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, 10032, New York, NY, U.S.A aut Cavanagh John NMR Structural Biology Facility, Wadsworth Center, New York State Department of Health, P.O. Box 509, 12201-0509, Albany, NY, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/1(1997-01-01), 94-100 0925-2738 9:1<94 1997 9 10858 https://doi.org/10.1023/A:1018679819693 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018679819693 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bracken Clay Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, 10032, New York, NY, U.S.A aut NATIONALLICENCE 700 1- III Arthur Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, 10032, New York, NY, U.S.A aut NATIONALLICENCE 700 1- Cavanagh John NMR Structural Biology Facility, Wadsworth Center, New York State Department of Health, P.O. Box 509, 12201-0509, Albany, NY, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/1(1997-01-01), 94-100 0925-2738 9:1<94 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer