caa a22 4500 47713629X CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1018671517876 doi (NATIONALLICENCE)springer-10.1023/A:1018671517876 Three-dimensional heteronuclear NMR techniques for assignment and conformational analysis using exchangeable protons in uniformly 13C-enriched oligosaccharides [Elektronische Daten] [R. Harris, T.J. Rutherford, M.J. Milton, S.W. Homans] We present heteronuclear three-dimensional gradient-NMR techniques for the resonanceassignment of exchangeable (-OH and -NH) protons in uniformly 13C isotopically enrichedoligosaccharides and for the measurement of 1H-1H nuclear Overhauser enhancementsinvolving these protons. These techniques are derived from conventional HOHAHA-HSQCand NOESY(ROESY)-HSQC experiments, and are illustrated in application to a sample ofuniformly 13C-enriched Galβ1-4GlcNAc, and demonstrate that a total of 35 ROEs involvingexchangeable protons can be detected and assigned. We present a quantitative analysis ofthese ROEs that can only be accommodated in a model of the solution behaviour of theoligosaccharide that involves considerable internal motion. Kluwer Academic Publishers, 1997 Oligosaccharide nationallicence 3D heteronuclear NMR nationallicence N-Acetyllactosamine nationallicence Harris R. Centre for Biomolecular Sciences, University of St. Andrews, The Purdie Building, KY16 9ST, St. Andrews, U.K aut Rutherford T.J. Centre for Biomolecular Sciences, University of St. Andrews, The Purdie Building, KY16 9ST, St. Andrews, U.K aut Milton M.J. Centre for Biomolecular Sciences, University of St. Andrews, The Purdie Building, KY16 9ST, St. Andrews, U.K aut Homans S.W. Centre for Biomolecular Sciences, University of St. Andrews, The Purdie Building, KY16 9ST, St. Andrews, U.K aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/1(1997-01-01), 47-54 0925-2738 9:1<47 1997 9 10858 https://doi.org/10.1023/A:1018671517876 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018671517876 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harris R. Centre for Biomolecular Sciences, University of St. Andrews, The Purdie Building, KY16 9ST, St. Andrews, U.K aut NATIONALLICENCE 700 1- Rutherford T.J. Centre for Biomolecular Sciences, University of St. Andrews, The Purdie Building, KY16 9ST, St. Andrews, U.K aut NATIONALLICENCE 700 1- Milton M.J. Centre for Biomolecular Sciences, University of St. Andrews, The Purdie Building, KY16 9ST, St. Andrews, U.K aut NATIONALLICENCE 700 1- Homans S.W. Centre for Biomolecular Sciences, University of St. Andrews, The Purdie Building, KY16 9ST, St. Andrews, U.K aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/1(1997-01-01), 47-54 0925-2738 9:1<47 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer