caa a22 4500 477136338 CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1023/A:1018683920602 doi (NATIONALLICENCE)springer-10.1023/A:1018683920602 Application of multiple-quantum line narrowing with simultaneous 1H and 13C constant-time scalar-coupling evolution in PFG-HACANH and PFG-HACA(CO)NH triple-resonance experiments [Elektronische Daten] [G.V.T. Swapna, Carlos Rios, Zhigang Shang, Gaetano Montelione] Many triple-resonance experiments make use of one-bond heteronuclear scalar couplings toestablish connectivities among backbone and/or side-chain nuclei. In medium-sized(15-30 kDa) proteins, short transverse relaxation times of Cα single-quantum stateslimit signal-to-noise (S/N) ratios. These relaxation properties can be improved usingheteronuclear multiple-quantum coherences (HMQCs) instead of heteronuclear single-quantumcoherences (HSQCs) in the pulse sequence design. In slowly tumbling macromolecules, theseHMQCs can exhibit significantly better transverse relaxation properties than HSQCs.However, HMQC-type experiments also exhibit resonance splittings due to multiple two- andthree-bond homo- and heteronuclear scalar couplings. We describe here a family of pulsed-field gradient (PFG) HMQC-type triple-resonance experiments using simultaneous 1H and13C constant-time (CT) periods to eliminate the t1 dependence of these scalar couplingeffects. These simultaneous CT PFG-(HA)CANH and PFG-(HA)CA(CO)NH HMQC-typeexperiments exhibit sharper resonance line widths and often have better S/N ratios than thecorresponding HSQC-type experiments. Results on proteins ranging in size from 6 to 30 kDashow average methine CαH HMQC:HSQC enhancement factors of 1.10 ± 0.15, withabout 40% of the cross peaks exhibiting better S/N ratios in the simultaneous CT-HMQCversions compared with the HSQC versions. Kluwer Academic Publishers, 1997 Heteronuclear relaxation nationallicence Heteronuclear multiple-quantum coherence nationallicence Bovine pancreatic trypsin inhibitor nationallicence Bovine pancreatic ribonuclease A nationallicence Influenza virus A NS-1 protein nationallicence BPTI-trypsin complex nationallicence Swapna G.V.T. Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, 679 Hoes Lane, 08854-5638, Piscataway, NJ, U.S.A aut Rios Carlos Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, 679 Hoes Lane, 08854-5638, Piscataway, NJ, U.S.A aut Shang Zhigang Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, 679 Hoes Lane, 08854-5638, Piscataway, NJ, U.S.A aut Montelione Gaetano Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, 679 Hoes Lane, 08854-5638, Piscataway, NJ, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/1(1997-01-01), 105-111 0925-2738 9:1<105 1997 9 10858 https://doi.org/10.1023/A:1018683920602 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018683920602 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Swapna G.V.T. Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, 679 Hoes Lane, 08854-5638, Piscataway, NJ, U.S.A aut NATIONALLICENCE 700 1- Rios Carlos Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, 679 Hoes Lane, 08854-5638, Piscataway, NJ, U.S.A aut NATIONALLICENCE 700 1- Shang Zhigang Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, 679 Hoes Lane, 08854-5638, Piscataway, NJ, U.S.A aut NATIONALLICENCE 700 1- Montelione Gaetano Center for Advanced Biotechnology and Medicine and Department of Molecular Biology and Biochemistry, Rutgers University, 679 Hoes Lane, 08854-5638, Piscataway, NJ, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/1(1997-01-01), 105-111 0925-2738 9:1<105 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer