caa a22 4500 477136354 CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1018643312309 doi (NATIONALLICENCE)springer-10.1023/A:1018643312309 Isotropic solutions of phospholipid bicelles: A new membrane mimetic for high-resolution NMR studies of polypeptides [Elektronische Daten] [Regitze Vold, R. Prosser, Alan Deese] In order to illustrate the utility of phospholipid bicelles [Sanders, C.R. and Schwonek, J.P.(1992) Biochemistry, 31, 8898-8905] as a membrane mimetic for high-resolution NMRstudies, we have recorded two-dimensional 1H NMR spectra of the tetradecameric peptidemastoparan Vespula lewisii in an isotropic aqueous solution of dimyristoyl and dihexanoylphosphatidylcholine. Mastoparan is largely unstructured in water, but assumes a well-definedhelical conformation in association with the bilayers. A pronounced periodicity of thesequential NH chemical shifts provides strong evidence that the helix axis of this shortpeptide is parallel, rather than perpendicular, to the bilayer plane. The bicellar solutions stillrequire in-depth morphological characterization, but they appear to be ideal media for NMRdetermination of the mode of binding and the structure of membrane-associated peptides andproteins. Kluwer Academic Publishers, 1997 Phospholipid bicelle nationallicence Membrane-bound peptide nationallicence Mastoparan nationallicence Vold Regitze Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, 92093-0359, La Jolla, CA, U.S.A aut Prosser R. Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, 92093-0359, La Jolla, CA, U.S.A aut Deese Alan Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, 92093-0359, La Jolla, CA, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 329-335 0925-2738 9:3<329 1997 9 10858 https://doi.org/10.1023/A:1018643312309 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018643312309 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vold Regitze Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, 92093-0359, La Jolla, CA, U.S.A aut NATIONALLICENCE 700 1- Prosser R. Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, 92093-0359, La Jolla, CA, U.S.A aut NATIONALLICENCE 700 1- Deese Alan Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, 92093-0359, La Jolla, CA, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 329-335 0925-2738 9:3<329 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer