caa a22 4500 477136397 CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1018687127330 doi (NATIONALLICENCE)springer-10.1023/A:1018687127330 A 15n-filtered 2D 1H TOCSY experiment for assignment of aromatic ring resonances and selective identification of tyrosine ring resonances in proteins: Description and application to Photoactive Yellow Protein [Elektronische Daten] [Brian Whitehead, Marco Tessari, Petra Düx, Rolf Boelens, Robert Kaptein, Geerten Vuister] A new method to selectively detect the ring resonances of the aromatic residues in 15N-labelled proteins is presented. The experiment consists of a 2D 1H TOCSY sequence withremoval of the amide signals via 15N-filtering. Experiments are acquired in the absence andpresence of water inversion; combining the two spectra allows selective observation of thetyrosine ring resonances and enables the identification of their δ andε ring protons. The experiment is demonstrated on a 15N-labelled sample of Photoactive Yellow Protein and isshown to give good selectivity for tyrosine ring resonances under a wide range oftemperatures and pH values. Kluwer Academic Publishers, 1997 TOCSY experiment nationallicence Aromatic rings nationallicence Tyrosine residues nationallicence Solvent exchange nationallicence Isotope filtering nationallicence Whitehead Brian Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut Tessari Marco Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut Düx Petra Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut Boelens Rolf Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut Kaptein Robert Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut Vuister Geerten Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 313-316 0925-2738 9:3<313 1997 9 10858 https://doi.org/10.1023/A:1018687127330 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018687127330 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Whitehead Brian Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Tessari Marco Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Düx Petra Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Boelens Rolf Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Kaptein Robert Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Vuister Geerten Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 313-316 0925-2738 9:3<313 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer