caa a22 4500 477136400 CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1018622708674 doi (NATIONALLICENCE)springer-10.1023/A:1018622708674 Assignment methodology for larger RNA oligonucleotides: Application to an ATP-binding RNA aptamer [Elektronische Daten] [Thorsten Dieckmann, Juli Feigon] The use of uniform 13C,15N labeling in the NMR spectroscopic study of RNA structures hasgreatly facilitated the assignment process in small RNA oligonucleotides. For ribose spinsystem assignments, exploitation of these labels has followed previously developed methodsfor the study of proteins. However, for sequential assignment of the exchangeable andnonexchangeable protons of the nucleotides, it has been necessary to develop a variety of newNMR experiments. Even these are of limited utility in the unambiguous assignment of largerRNAs due to the short carbon relaxation times and extensive spectral overlap for all nuclei.These problems can largely be overcome by the additional use of base-type selectively13C,15N-labeled RNA in combination with a judicious use of related RNAs with basesubstitutions. We report the application of this approach to a 36-nucleotide ATP-binding RNAaptamer in complex with AMP. Complete sequential 1H assignments, as well as the majorityof 13C and 15N assignments, were obtained. Kluwer Academic Publishers, 1997 In vitro selection nationallicence AMP nationallicence Heteronuclear NMR nationallicence Specific labeling nationallicence RNA structure nationallicence Dieckmann Thorsten Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, P.O. Box 951569, 90095-1569, Los Angeles, CA, U.S.A aut Feigon Juli Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, P.O. Box 951569, 90095-1569, Los Angeles, CA, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 259-272 0925-2738 9:3<259 1997 9 10858 https://doi.org/10.1023/A:1018622708674 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018622708674 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dieckmann Thorsten Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, P.O. Box 951569, 90095-1569, Los Angeles, CA, U.S.A aut NATIONALLICENCE 700 1- Feigon Juli Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, P.O. Box 951569, 90095-1569, Los Angeles, CA, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 259-272 0925-2738 9:3<259 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer