caa a22 4500 477136443 CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1018691228238 doi (NATIONALLICENCE)springer-10.1023/A:1018691228238 χ1 angle information from a simple two-dimensional NMR experiment that identifies trans 3JNCγ couplings in isotopically enriched proteins [Elektronische Daten] [Jin-Shan Hu, Ad Bax] New quantitative J correlation experiments are used for measuring all two- and three-bondcouplings between 15N and aliphatic side-chain carbons in proteins uniformly enriched in 13Cand 15N. Results show that 3JNCβ and 2JNCβ invariably are very small.Therefore, a simple and relatively sensitive two-dimensional spin-echo difference experimentcan be used to identify residues with a 3JNCγ coupling substantially larger than 1Hz, indicative of a trans arrangement between N and Cγ. This measurement thereforeprovides χ1 angle information for residues with an aliphatic Cγ carbon, andthereby also aids in making stereospecific assignments of Hβ resonances. Experimentsare demonstrated for ubiquitin and for a complex between calmodulin and a 26-residuepeptide. Kluwer Academic Publishers, 1997 χ1 angle nationallicence Calmodulin nationallicence Carbon-nitrogen J coupling nationallicence Quantitative J correlation nationallicence Stereospecific assignment nationallicence Ubiquitin nationallicence Hu Jin-Shan Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892-0520, Bethesda, MD, U.S.A aut Bax Ad Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892-0520, Bethesda, MD, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 323-328 0925-2738 9:3<323 1997 9 10858 https://doi.org/10.1023/A:1018691228238 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018691228238 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Jin-Shan Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892-0520, Bethesda, MD, U.S.A aut NATIONALLICENCE 700 1- Bax Ad Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892-0520, Bethesda, MD, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 323-328 0925-2738 9:3<323 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer