caa a22 4500 477136451 CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970401xx s 000 0 eng 10.1023/A:1018678925512 doi (NATIONALLICENCE)springer-10.1023/A:1018678925512 NMR secondary structure of the plasminogen activator protein staphylokinase [Elektronische Daten] [O. Ohlenschläger, R. Ramachandran, J. Flemming, K.-H. Gührs, B. Schlott, L.R. Brown] Staphylokinase (Sak) is a 15.5 kDa protein secreted by several strains of Staphylococcusaureus. Due to its ability to convert plasminogen, the inactive proenzyme of the fibrinolyticsystem, into plasmin, Sak is presently undergoing clinical trials for blood clot lysis in thetreatment of thrombovascular disorders. With a view to developing a better understanding ofthe mode of action of Sak, we have initiated a structural investigation of Sak viamultidimensional heteronuclear NMR spectroscopy employing uniformly 15N- and 15N,13C-labelled Sak. Sequence-specific resonance assignments have been made employing 15N-editedTOCSY and NOE experiments and from HNCACB, CBCA(CO)NH, HBHA(CBCACO)NHand CC(CO)NH sets of experiments. From an analysis of the chemical shifts,3JHNHα scalar coupling constants, NOEs and HN exchange data, the secondary structural elements of Sakhave been characterized. Kluwer Academic Publishers, 1997 Secondary structure nationallicence NOE nationallicence Chemical shift nationallicence Heteronuclear NMR nationallicence Staphylokinase nationallicence Plasminogen nationallicence Resonance assignment nationallicence Ohlenschläger O. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut Ramachandran R. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut Flemming J. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut Gührs K.-H Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut Schlott B. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut Brown L.R. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 273-286 0925-2738 9:3<273 1997 9 10858 https://doi.org/10.1023/A:1018678925512 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018678925512 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohlenschläger O. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut NATIONALLICENCE 700 1- Ramachandran R. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut NATIONALLICENCE 700 1- Flemming J. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut NATIONALLICENCE 700 1- Gührs K.-H Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut NATIONALLICENCE 700 1- Schlott B. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut NATIONALLICENCE 700 1- Brown L.R. Department of Molecular Biophysics/NMR Spectroscopy, Institute of Molecular Biotechnology, P.O. Box 100813, D-07708, Jena, Germany aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/3(1997-04-01), 273-286 0925-2738 9:3<273 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer