caa a22 4500 477136486 CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970601xx s 000 0 eng 10.1023/A:1018311013338 doi (NATIONALLICENCE)springer-10.1023/A:1018311013338 Improved labeling strategy for 13C relaxation measurements of methyl groups in proteins [Elektronische Daten] [Andrew Lee, Jeffrey Urbauer, A. Wand] Selective incorporation of 13C into the methyl groupsof protein side chains is described as a means for simplifying themeasurement and interpretation of 13C relaxation parameters.High incorporation (>90%) is accomplished by using pyruvate(3-13C, 99%) as the sole carbon source in the growthmedia for protein overexpression in E. coli. This improved labeling schemeincreases the sensitivity of the relaxation experiments by approximatelyfivefold when compared to randomly fractionally 13C-labeledprotein, allowing high-quality measurements on relatively dilute (<1 mM)protein samples at a relatively low cost. Kluwer Academic Publishers, 1997 Relaxation nationallicence Methyl nationallicence 13C nationallicence Protein nationallicence Dynamics nationallicence Lee Andrew Departments of Biological Sciences, Biophysical Sciences and Chemistry, and Center for Structural Biology, State University of New York at Buffalo, 816 Natural Sciences and Mathematics Complex, 14260-3000, Buffalo, NY, U.S.A aut Urbauer Jeffrey Departments of Biological Sciences, Biophysical Sciences and Chemistry, and Center for Structural Biology, State University of New York at Buffalo, 816 Natural Sciences and Mathematics Complex, 14260-3000, Buffalo, NY, U.S.A aut Wand A. Departments of Biological Sciences, Biophysical Sciences and Chemistry, and Center for Structural Biology, State University of New York at Buffalo, 816 Natural Sciences and Mathematics Complex, 14260-3000, Buffalo, NY, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/4(1997-06-01), 437-440 0925-2738 9:4<437 1997 9 10858 https://doi.org/10.1023/A:1018311013338 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018311013338 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Andrew Departments of Biological Sciences, Biophysical Sciences and Chemistry, and Center for Structural Biology, State University of New York at Buffalo, 816 Natural Sciences and Mathematics Complex, 14260-3000, Buffalo, NY, U.S.A aut NATIONALLICENCE 700 1- Urbauer Jeffrey Departments of Biological Sciences, Biophysical Sciences and Chemistry, and Center for Structural Biology, State University of New York at Buffalo, 816 Natural Sciences and Mathematics Complex, 14260-3000, Buffalo, NY, U.S.A aut NATIONALLICENCE 700 1- Wand A. Departments of Biological Sciences, Biophysical Sciences and Chemistry, and Center for Structural Biology, State University of New York at Buffalo, 816 Natural Sciences and Mathematics Complex, 14260-3000, Buffalo, NY, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/4(1997-06-01), 437-440 0925-2738 9:4<437 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer