caa a22 4500 477136508 CHVBK 20180405111719.0 cr unu---uuuuu 170330e19970601xx s 000 0 eng 10.1023/A:1018369721091 doi (NATIONALLICENCE)springer-10.1023/A:1018369721091 Structural features of the metal binding site and dynamics of gallium putidaredoxin, a diamagnetic derivative of a Cys4Fe2S2ferredoxin [Elektronische Daten] [Sophia Kazanis, Thomas Pochapsky] The first reconstitution of an Fe2S2ferredoxin with a diamagnetic prosthetic group was recently described[Kazanis et al. (1995) J. Am. Chem. Soc., 117, 6625-6626]. Thereplacement of the iron-sulfur cluster of the bacterial ferredoxinputidaredoxin (Pdx) by gallium (Ga3+) renders the proteindiamagnetic and permits the use of high-resolution NMR methods to identifyresonances near the metal binding site. We now describe structural featuresof the metal binding site that are not observable by standard NMR methods innative Pdx due to paramagnetic line broadening. These results provide thefirst example of high-resolution NMR-derived structural data concerning themetal binding domain of an Fe2S2 ferredoxin, andthe first structural information of any sort for the metal binding site of aferredoxin from this class, which includes adrenodoxin, placental ferredoxinand terpredoxin. Assignments were obtained by applying multidimensional NMRmethods to a series of selectively and nonselectively 15N- and13C/15N-labeled GaPdx samples. For mostexperiments, a mutant of Pdx was used in which a nonligatingCys85 is replaced by serine. All of the major structuralfeatures that were identified in native Pdx are conserved in GaPdx. Theoverall protein dynamics is considerably faster in GaPdx than in the nativeprotein, as reflected by amide proton exchange rates. The C-terminalresidue, Trp106, also exhibits considerable mobility, asindicated by 15N{1H} NOE and 15NT1 values of the C-terminal residue of the protein. Kluwer Academic Publishers, 1997 Electron transfer nationallicence Putidaredoxin nationallicence Cytochrome P450 nationallicence Metalloprotein nationallicence Gallium nationallicence Kazanis Sophia Bioorganic Chemistry Program, Brandeis University, 02254-9110, Waltham, MA, U.S.A aut Pochapsky Thomas Department of Chemistry, Brandeis University, 02254-9110, Waltham, MA, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/4(1997-06-01), 337-346 0925-2738 9:4<337 1997 9 10858 https://doi.org/10.1023/A:1018369721091 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018369721091 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kazanis Sophia Bioorganic Chemistry Program, Brandeis University, 02254-9110, Waltham, MA, U.S.A aut NATIONALLICENCE 700 1- Pochapsky Thomas Department of Chemistry, Brandeis University, 02254-9110, Waltham, MA, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/4(1997-06-01), 337-346 0925-2738 9:4<337 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer