caa a22 4500 477136575 CHVBK 20180405111720.0 cr unu---uuuuu 170330e19970601xx s 000 0 eng 10.1023/A:1018354712430 doi (NATIONALLICENCE)springer-10.1023/A:1018354712430 Pulse schemes for the measurement of3 JC′Cγ and3 JNCγ scalar couplings in 15N,13C uniformly labeled proteins [Elektronische Daten] [Robert Konrat, D.R. Muhandiram, N.A. Farrow, Lewis Kay] Pulse sequences are presented for the measurement of3JC′Cγ and3JNCγ scalar couplings for allCγ containing residues in15N,13C uniformly labeled proteins. The methodsdescribed are based on quantitative J correlation spectroscopy pioneered byBax and co-workers [Bax et al. (1994) Methods Enzymol., 239, 79-105].The combination of 3JC′Cγ and3JNCγ scalar coupling constants allows theassignment of discrete rotameric states about the χ1 torsion angle in cases where such states exist or, alternatively,facilitates the establishment of noncanonical χ1conformations or the presence of rotameric averaging. The methods areapplied to a 1.5 mM sample of staphylococcal nuclease. Kluwer Academic Publishers, 1997 Triple-resonance NMR nationallicence Quantitative J spectroscopy nationallicence 3JC′Cγ and 3JNCγ scalar couplings nationallicence χ1 dihedral angle nationallicence Konrat Robert Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, M5S 1A8, Toronto, ON, Canada aut Muhandiram D.R. Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, M5S 1A8, Toronto, ON, Canada aut Farrow N.A. Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, M5S 1A8, Toronto, ON, Canada aut Kay Lewis Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, M5S 1A8, Toronto, ON, Canada aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/4(1997-06-01), 409-422 0925-2738 9:4<409 1997 9 10858 https://doi.org/10.1023/A:1018354712430 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018354712430 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Konrat Robert Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, M5S 1A8, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Muhandiram D.R. Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, M5S 1A8, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Farrow N.A. Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, M5S 1A8, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Kay Lewis Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, M5S 1A8, Toronto, ON, Canada aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/4(1997-06-01), 409-422 0925-2738 9:4<409 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer