caa a22 4500 477136591 CHVBK 20180405111720.0 cr unu---uuuuu 170330e19970201xx s 000 0 eng 10.1023/A:1018698002314 doi (NATIONALLICENCE)springer-10.1023/A:1018698002314 Two-dimensional 1H NMR experiments show that the 23-residue magainin antibiotic peptide is an α-helix in dodecylphosphocholine micelles, sodium dodecylsulfate micelles, and trifluoroethanol/water solution [Elektronische Daten] [Jennifer Gesell, Michael Zasloff, Stanley Opella] Magainin2 is a 23-residue antibiotic peptide that disrupts the ionic gradient across certain cellmembranes. Two-dimensional 1H NMR spectroscopy was used to investigate the structure ofthe peptide in three of the membrane environments most commonly employed in biophysicalstudies. Sequence-specific resonance assignments were determined for the peptide inperdeuterated dodecylphosphocholine (DPC) and sodium dodecylsulfate micelles andconfirmed for the peptide in 2,2,2-trifluoroethanol solution. The secondary structure is shownto be helical in all of the solvent systems. The NMR data were used as a set of restraints fora simulated annealing protocol that generated a family of three-dimensional structures of thepeptide in DPC micelles, which superimposed best between residues 4 and 20. For theseresidues, the mean pairwise rms difference for the backbone atoms is 0.47 ± 0.10Å from the average structure. The calculated peptide structures appear to be curved,with the bend centered at residues Phe12 and Gly13. Kluwer Academic Publishers, 1997 Magainin nationallicence Membrane nationallicence Amphipathic helix nationallicence Micelle nationallicence Gesell Jennifer Department of Chemistry, University of Pennsylvania, 19104, Philadelphia, PA, U.S.A aut Zasloff Michael Magainin Pharmaceuticals Inc., 1550 Campus Drive, 19355, Plymouth Meeting, PA, U.S.A aut Opella Stanley Department of Chemistry, University of Pennsylvania, 19104, Philadelphia, PA, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/2(1997-02-01), 127-135 0925-2738 9:2<127 1997 9 10858 https://doi.org/10.1023/A:1018698002314 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018698002314 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gesell Jennifer Department of Chemistry, University of Pennsylvania, 19104, Philadelphia, PA, U.S.A aut NATIONALLICENCE 700 1- Zasloff Michael Magainin Pharmaceuticals Inc., 1550 Campus Drive, 19355, Plymouth Meeting, PA, U.S.A aut NATIONALLICENCE 700 1- Opella Stanley Department of Chemistry, University of Pennsylvania, 19104, Philadelphia, PA, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/2(1997-02-01), 127-135 0925-2738 9:2<127 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer