caa a22 4500 47713663X CHVBK 20180405111720.0 cr unu---uuuuu 170330e19970201xx s 000 0 eng 10.1023/A:1018614505948 doi (NATIONALLICENCE)springer-10.1023/A:1018614505948 A three-dimensional NMR experiment with improved sensitivity for carbonyl-carbonyl J correlation in proteins [Elektronische Daten] [Stephan Grzesiek, Ad Bax] Recently, a quantitative J correlation technique has been presented that permits measurementof 3JC′C′ in proteins isotopically enriched with 13C [Hu, J.-S. and Bax, A.(1996) J. Am. Chem. Soc., 118, 8170-8171]. Here, we describe an analogousexperiment that is less sensitive to transverse 13C′ relaxation, which is the principallimiting factor in all 13C-13C long-range correlation experiments on macromolecules. Thenew scheme utilizes homonuclear Hartmann-Hahn cross polarization (TOCSY) insteadof a COSY-type transfer to accomplish magnetization transfer; a description of the relevantrelaxation terms is presented. The experiment is demonstrated for ubiquitin and HIV-1 Nef.The results show excellent agreement between 3JC′C′ values measured forubiquitin with the new scheme and those reported previously. The experiment is particularlyuseful for distinguishing backbone φ angles that are smaller than -120° from thoselarger than -120°. Kluwer Academic Publishers, 1997 Carbon-carbon J coupling nationallicence HOHAHA nationallicence TOCSY nationallicence Relaxation nationallicence φ Angle nationallicence HIV-1 Nef nationallicence Ubiquitin nationallicence Grzesiek Stephan Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892-0520, Bethesda, MD, U.S.A aut Bax Ad Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892-0520, Bethesda, MD, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/2(1997-02-01), 207-211 0925-2738 9:2<207 1997 9 10858 https://doi.org/10.1023/A:1018614505948 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018614505948 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grzesiek Stephan Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892-0520, Bethesda, MD, U.S.A aut NATIONALLICENCE 700 1- Bax Ad Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892-0520, Bethesda, MD, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/2(1997-02-01), 207-211 0925-2738 9:2<207 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer