caa a22 4500 477136648 CHVBK 20180405111720.0 cr unu---uuuuu 170330e19970201xx s 000 0 eng 10.1023/A:1018606304131 doi (NATIONALLICENCE)springer-10.1023/A:1018606304131 1h, 13c, and 15N NMR backbone assignments and chemical-shift-derived secondary structure of glutamine-binding protein of Escherichia coli [Elektronische Daten] [Jinghua Yu, Virgil Simplaceanu, Nico Tjandra, Patricia Cottam, Jonathan Lukin, Chien Ho] 1H, 13C, and 15N NMR assignments of the backbone atoms and β-carbons have been madefor liganded glutamine-binding protein (GlnBP) of Escherichia coli, a monomeric protein with226 amino acid residues and a molecular weight of 24,935 Da. GlnBP is a periplasmicbinding protein which plays an essential role in the active transport of L-glutamine throughthe cytoplasmic membrane. The assignments have been obtained from three-dimensionaltriple-resonance NMR experiments on a 13C,15N uniformly labeled sample as well asspecifically labeled samples. Results from the 3D triple-resonance experiments, HNCO,HN(CO)CA, HN(COCA)HA, HNCA, HN(CA)HA, HN(CA)CO, and CBCA(CO)NH, are themain sources used to make the resonance assignments. Other 3D experiments, such asHNCACB, COCAH, HCACO, HCACON, and HOHAHA-HMQC, have been used to confirmthe resonance assignments and to extend connections where resonance peaks are missing insome of the experiments mentioned above. We have assigned more than 95% of thepolypeptide backbone resonances of GlnBP. The result of the standard manual assignment isin agreement with that predicted by an automated probabilistic method developed in ourlaboratory. A solution secondary structure of the GlnBP-Gln complex has beenproposed based on chemical shift deviations from random coil values. Eight α-helices and10 β-strands are derived using the Chemical Shift Index method. Kluwer Academic Publishers, 1997 Triple-resonance NMR nationallicence Backbone assignment nationallicence Glutamine-binding protein nationallicence Yu Jinghua Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut Simplaceanu Virgil Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut Tjandra Nico Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut Cottam Patricia Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut Lukin Jonathan Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut Ho Chien Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/2(1997-02-01), 167-180 0925-2738 9:2<167 1997 9 10858 https://doi.org/10.1023/A:1018606304131 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018606304131 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Jinghua Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut NATIONALLICENCE 700 1- Simplaceanu Virgil Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut NATIONALLICENCE 700 1- Tjandra Nico Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut NATIONALLICENCE 700 1- Cottam Patricia Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut NATIONALLICENCE 700 1- Lukin Jonathan Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut NATIONALLICENCE 700 1- Ho Chien Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, 15213-2683, Pittsburgh, PA, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/2(1997-02-01), 167-180 0925-2738 9:2<167 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer