caa a22 4500 477136656 CHVBK 20180405111720.0 cr unu---uuuuu 170330e19970201xx s 000 0 eng 10.1023/A:1018662421878 doi (NATIONALLICENCE)springer-10.1023/A:1018662421878 Selective 15N labeling and direct observation by NMR of the active-site glutamine of Fe-containing superoxide dismutase [Elektronische Daten] [Carrie Vance, Young Kang, Anne-Frances Miller] The glutamine in position 69 is one of only three conserved active-site amino acid differencesbetween Fe- and Mn-containing superoxide dismutases (SODs). We have refined theconditions for extremely selective labeling of the side chains of glutamine with 15N, and thusobtained dramatically simplified spectra, despite the large size of Fe-SOD. The improvedresolution afforded by such highly specific labeling permits the use of direct 15N detectionto observe and assign Gln 69, even though its distance to the paramagnetic Fe2+ is only 5Å. Selective glutamine side-chain labeling is inexpensive and has general utility forlarge (and paramagnet-containing) proteins. Kluwer Academic Publishers, 1997 Selective labeling nationallicence Glutamine nationallicence 15N nationallicence Superoxide dismutase nationallicence Vance Carrie Departments of Biophysics and Chemistry, The Johns Hopkins University, 3400 N. Charles Street, 21218, Baltimore, MD, U.S.A aut Kang Young Departments of Biophysics and Chemistry, The Johns Hopkins University, 3400 N. Charles Street, 21218, Baltimore, MD, U.S.A aut Miller Anne-Frances Departments of Biophysics and Chemistry, The Johns Hopkins University, 3400 N. Charles Street, 21218, Baltimore, MD, U.S.A aut Journal of Biomolecular NMR Kluwer Academic Publishers 9/2(1997-02-01), 201-206 0925-2738 9:2<201 1997 9 10858 https://doi.org/10.1023/A:1018662421878 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1018662421878 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vance Carrie Departments of Biophysics and Chemistry, The Johns Hopkins University, 3400 N. Charles Street, 21218, Baltimore, MD, U.S.A aut NATIONALLICENCE 700 1- Kang Young Departments of Biophysics and Chemistry, The Johns Hopkins University, 3400 N. Charles Street, 21218, Baltimore, MD, U.S.A aut NATIONALLICENCE 700 1- Miller Anne-Frances Departments of Biophysics and Chemistry, The Johns Hopkins University, 3400 N. Charles Street, 21218, Baltimore, MD, U.S.A aut NATIONALLICENCE 773 0- Journal of Biomolecular NMR Kluwer Academic Publishers 9/2(1997-02-01), 201-206 0925-2738 9:2<201 1997 9 10858 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer