caa a22 4500 477136907 CHVBK 20180405111721.0 cr unu---uuuuu 170330e19970101xx s 000 0 eng 10.1007/BF00127966 doi (NATIONALLICENCE)springer-10.1007/BF00127966 Effect of acetylation and permethylation on the conformation and candidacidal activity of salivary histatin-5 [Elektronische Daten] [Kalaiyarasi Ramalingam, Narayanan Ramasubbu, Michael Levine] Summary: Salivary histatins provide the non-immune defense against oral pathogens such as Candida albicans. The structural requirements of histatin-5 for anticandida activity were examined with respect to its ability to adopt helical structures, its electrostatic interactions and the hydrogen-bonding potency of its basic residues. For this purpose, the lysine and/or histidine residues of histatin-5 were chemically modified by acetylation and permethylation. Acetylated histatin-5 retained its ability to adopt helical structures in 2,2,2-trifluoroethanol, but completely lost its ability to kill yeast cells. In contrast, permethylated histatin-5 shows very little tendency to adopt a helical structure, but retained significant anticandida activity. The results suggest that the candidacidal activity can arise even when the histatin does not have the ability to adopt helical structures. The candidacidal activity of the derivatives is discussed in terms of electrostatic interactions and hydrogen-bonding potency. ESCOM Science Publishers B.V., 1997 Histatins nationallicence Peptide synthesis nationallicence Candida albicans nationallicence Structure nationallicence Permethylation nationallicence Ramalingam Kalaiyarasi Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, 109 Foster Hall, 14214, Buffalo, NY, U.S.A. aut Ramasubbu Narayanan Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, 109 Foster Hall, 14214, Buffalo, NY, U.S.A. aut Levine Michael Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, 109 Foster Hall, 14214, Buffalo, NY, U.S.A. aut Letters in Peptide Science Kluwer Academic Publishers 3/6(1997-01-01), 349-356 0929-5666 3:6<349 1997 3 10989 https://doi.org/10.1007/BF00127966 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00127966 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ramalingam Kalaiyarasi Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, 109 Foster Hall, 14214, Buffalo, NY, U.S.A aut NATIONALLICENCE 700 1- Ramasubbu Narayanan Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, 109 Foster Hall, 14214, Buffalo, NY, U.S.A aut NATIONALLICENCE 700 1- Levine Michael Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, 109 Foster Hall, 14214, Buffalo, NY, U.S.A aut NATIONALLICENCE 773 0- Letters in Peptide Science Kluwer Academic Publishers 3/6(1997-01-01), 349-356 0929-5666 3:6<349 1997 3 10989 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer