naa a22 4500 510724787 CHVBK 20180411082931.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s11947-012-0876-8 doi (NATIONALLICENCE)springer-10.1007/s11947-012-0876-8 Determination of Ochratoxin A in Bread: Evaluation of Microwave-Assisted Extraction Using an Orthogonal Composite Design Coupled with Response Surface Methodology [Elektronische Daten] [Paula Paíga, Simone Morais, Teresa Oliva-Teles, Manuela Correia, Cristina Delerue-Matos, Ana Sousa, Maria Gonçalves, Sofia Duarte, Angelina Pena, Celeste Lino] An analytical method using microwave-assisted extraction (MAE) and liquid chromatography (LC) with fluorescence detection (FD) for the determination of ochratoxin A (OTA) in bread samples is described. A 24 orthogonal composite design coupled with response surface methodology was used to study the influence of MAE parameters (extraction time, temperature, solvent volume, and stirring speed) in order to maximize OTA recovery. The optimized MAE conditions were the following: 25mL of acetonitrile, 10min of extraction, at 80°C, and maximum stirring speed. Validation of the overall methodology was performed by spiking assays at five levels (0.1-3.00ng/g). The quantification limit was 0.005ng/g. The established method was then applied to 64 bread samples (wheat, maize, and wheat/maize bread) collected in Oporto region (Northern Portugal). OTA was detected in 84% of the samples with a maximum value of 2.87ng/g below the European maximum limit established for OTA in cereal products of 3ng/g. Springer Science+Business Media, LLC, 2012 Ochratoxin A nationallicence Liquid chromatography nationallicence Microwave assisted extraction nationallicence Bread nationallicence Paíga Paula REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut Morais Simone REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut Oliva-Teles Teresa REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut Correia Manuela REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut Delerue-Matos Cristina REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut Sousa Ana REQUIMTE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal aut Gonçalves Maria REQUIMTE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal aut Duarte Sofia Group of Health Surveillance, Center of Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Health Sciences Campus, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal aut Pena Angelina Group of Health Surveillance, Center of Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Health Sciences Campus, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal aut Lino Celeste Group of Health Surveillance, Center of Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Health Sciences Campus, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal aut Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/9(2013-09-01), 2466-2477 1935-5130 6:9<2466 2013 6 11947 https://doi.org/10.1007/s11947-012-0876-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-012-0876-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paíga Paula REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut NATIONALLICENCE 700 1- Morais Simone REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut NATIONALLICENCE 700 1- Oliva-Teles Teresa REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut NATIONALLICENCE 700 1- Correia Manuela REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut NATIONALLICENCE 700 1- Delerue-Matos Cristina REQUIMTE, Instituto Superior de Engenharia do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072, Porto, Portugal aut NATIONALLICENCE 700 1- Sousa Ana REQUIMTE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal aut NATIONALLICENCE 700 1- Gonçalves Maria REQUIMTE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal aut NATIONALLICENCE 700 1- Duarte Sofia Group of Health Surveillance, Center of Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Health Sciences Campus, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal aut NATIONALLICENCE 700 1- Pena Angelina Group of Health Surveillance, Center of Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Health Sciences Campus, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal aut NATIONALLICENCE 700 1- Lino Celeste Group of Health Surveillance, Center of Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Health Sciences Campus, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/9(2013-09-01), 2466-2477 1935-5130 6:9<2466 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer