naa a22 4500 510725066 CHVBK 20180411082932.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s11947-011-0665-9 doi (NATIONALLICENCE)springer-10.1007/s11947-011-0665-9 Effect of Cleaning Treatment on Adhesion of Streptococcus agalactiae to Milking Machine Surfaces [Elektronische Daten] [Adbeel Santos, Ana Pires, José Behaine, Emiliane Araújo, Nélio de Andrade, Antônio de Carvalho] Streptococcus agalactiae is a common subclinical mastitis agent in dairy cattle. In this study, the influence of hygiene procedure time on the adhesion of S. agalactiae to rubber and silicone surfaces used in milking machines was evaluated. The effect of hygiene practices on the thermodynamic and microscopic features of both surfaces was also evaluated. The hydrophobicity of milking machines and bacterial surfaces was investigated by measuring the contact angle with a goniometer 0, 30, 90, and 180days after a full hygiene procedure simulated using rubber and silicone coupons. As the time of cleaning procedures enhanced, there was a reduction in the adhesion of S. agalactiae to both surfaces. The rubber and silicone surfaces were hydrophobic in all treatments $$ \left( {\Delta G_{\text{sws}}^{\text{TOT}}\; < \;0} \right) $$ , while the bacterial surface presented hydrophilic behavior $$ \left( {\Delta G_{\text{sws}}^{\text{TOT}}\; > \;0} \right) $$ , which makes the adhesion process difficult. Photomicrographs showed rapid wear of both surfaces, pointing to damages caused by cleaning agents. However, the silicone was more resistant to cleaning and sanitizing treatments. Thus, this work shows that changes caused by hygiene procedures in the thermodynamic and in the morphology of milking surfaces have an enormous importance in the S. agalactiae adhesion and, consequently, in mastitis transmission between cattle herd. Springer Science+Business Media, LLC, 2011 Biofilm nationallicence Mastitis nationallicence Hygiene nationallicence Hydrophobicity nationallicence Santos Adbeel Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), Campus UFV, 36570-000, Viçosa, Minas Gerais, Brazil aut Pires Ana Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil aut Behaine José Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil aut Araújo Emiliane Universidade Federal de Viçosa, Campus Rio Paranaíba, 38810-000, Rio Paranaíba, Minas Gerais, Brazil aut de Andrade Nélio Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil aut de Carvalho Antônio Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil aut Food and Bioprocess Technology Springer-Verlag 6/7(2013-07-01), 1868-1872 1935-5130 6:7<1868 2013 6 11947 https://doi.org/10.1007/s11947-011-0665-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-011-0665-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santos Adbeel Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), Campus UFV, 36570-000, Viçosa, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- Pires Ana Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- Behaine José Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- Araújo Emiliane Universidade Federal de Viçosa, Campus Rio Paranaíba, 38810-000, Rio Paranaíba, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- de Andrade Nélio Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- de Carvalho Antônio Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer-Verlag 6/7(2013-07-01), 1868-1872 1935-5130 6:7<1868 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer