naa a22 4500 510725120 CHVBK 20180411082932.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s11947-012-0814-9 doi (NATIONALLICENCE)springer-10.1007/s11947-012-0814-9 Dehydrogenase Enzymes Associated to Glycolysis in Beef Carcasses Stored at 0°C [Elektronische Daten] [Cristian Acevedo, María Cornejo, Yusser Olguín, Ronny Vallejos, Donald Brown] After the death of an animal, cell metabolism is controlled locally. The post-mortem oxygen depletion increases the glycolytic activity and lactate production. However, many mechanisms of post-mortem metabolic regulation have not been fully investigated in beef carcasses. In this work, we studied the post-mortem glycolytic behavior (including lactate dehydrogenase) and three dehydrogenase associated to glycolysis (glycerophosphate dehydrogenase, glucose 6-phosphate dehydrogenase, and glycerol dehydrogenase) by using cytochemistry techniques in three fast-twitch muscles (M. longissimus dorsi, M. semimembranosus, and M. cutaneus trunci) of carcasses stored at 0°C. Our results indicate that glycolysis depends on the type of muscle. The post-mortem glycolytic flux and lactate dehydrogenase activity of M. cutaneus trunci was the lowest of the three muscles studied. Of the other dehydrogenases analyzed, only glycerophosphate and glycerol dehydrogenase showed clear cytochemical reaction. Glucose 6-phosphate dehydrogenase was not used by muscles very much. The glycerophosphate dehydrogenase was the strongest enzymatic activity correlated to the post-mortem glycolytic flux. In addition, a relationship between glycerophosphate dehydrogenase and glycerol dehydrogenase was detected by using a multiple regression model. This phenomenon was studied by using bioinformatics tools, suggesting that glycerophosphate dehydrogenase could oxidize the glycerol in bovine fast-twitch muscles. Springer Science+Business Media, LLC, 2012 Beef nationallicence Carcasses nationallicence Dehydrogenases nationallicence Enzymes nationallicence Glycolysis nationallicence Acevedo Cristian Universidad Técnica Federico Santa María. Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile aut Cornejo María Departamento de Biología y Ciencias Ambientales, Universidad de Valparaíso. Facultad de Ciencias, Avenida Gran Bretaña 1111, Valparaíso, Chile aut Olguín Yusser Universidad Técnica Federico Santa María. Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile aut Vallejos Ronny Departamento de Matemática, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile aut Brown Donald Departamento de Biología y Ciencias Ambientales, Universidad de Valparaíso. Facultad de Ciencias, Avenida Gran Bretaña 1111, Valparaíso, Chile aut Food and Bioprocess Technology Springer-Verlag 6/7(2013-07-01), 1696-1702 1935-5130 6:7<1696 2013 6 11947 https://doi.org/10.1007/s11947-012-0814-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-012-0814-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Acevedo Cristian Universidad Técnica Federico Santa María. Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile aut NATIONALLICENCE 700 1- Cornejo María Departamento de Biología y Ciencias Ambientales, Universidad de Valparaíso. Facultad de Ciencias, Avenida Gran Bretaña 1111, Valparaíso, Chile aut NATIONALLICENCE 700 1- Olguín Yusser Universidad Técnica Federico Santa María. Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile aut NATIONALLICENCE 700 1- Vallejos Ronny Departamento de Matemática, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile aut NATIONALLICENCE 700 1- Brown Donald Departamento de Biología y Ciencias Ambientales, Universidad de Valparaíso. Facultad de Ciencias, Avenida Gran Bretaña 1111, Valparaíso, Chile aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer-Verlag 6/7(2013-07-01), 1696-1702 1935-5130 6:7<1696 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer