naa a22 4500 51072521X CHVBK 20180411082932.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11947-012-1008-1 doi (NATIONALLICENCE)springer-10.1007/s11947-012-1008-1 Aqueous Two-Phase Micellar System for Nisin Extraction in the Presence of Electrolytes [Elektronische Daten] [Angela Jozala, André Lopes, Letícia de Lencastre Novaes, Priscila Mazzola, Thereza Penna, Adalberto Júnior] Liquid-liquid extraction for aqueous two-phase micellar systems (ATPMS) is a promising technique that can either replace or be used as a complementary process to more typical chromatographic operations in order to reduce the costs of downstream processing of many biological products. This method offers attractive conditions when exploited in the extraction/purification of a target protein directly on the culture medium. Nisin, an extracellular antimicrobial peptide, is produced by Lactococcus lactis and is effective at controlling a wide range of Gram-positive bacteria, including multidrug-resistant pathogens. This study evaluates ATPMS composed by a nonionic surfactant, Triton X-114, in the presence or absence of electrolytes, to improve the extraction of nisin. The partitioning behavior of nisin showed that it can be directly extracted from the fermentation media. The partitioning coefficient (K nis) of the commercial nisin in the presence of electrolytes showed K nis values of 5.6 and 5.4 for MgSO4 and (NH4)2SO4, respectively. Similar behavior was observed for the produced nisin where K nis values were 4.1 and 5.1 for MgSO4 and (NH4)2SO4. After partition, the commercial nisin activity in the micelle-rich phase, in the absence of electrolytes, was 3.3 logAU/mL, and in the presence of MgSO4 and (NH4)2SO4, the activity was 5.0logAU/mL. The produced nisin activity with and without MgSO4 was around 3.5logAU/mL; however, in the presence of (NH4)2SO4, nisin activity was 4.5logAU/mL. The increase in nisin activity after partitioning with salts encourages further researches for the optimization of nisin extraction. Springer Science+Business Media New York, 2012 Liquid-liquid extraction nationallicence Electrolytes nationallicence Lactococcus lactis nationallicence Peptide purification nationallicence Nisin nationallicence Triton X-114 nationallicence Jozala Angela Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut Lopes André Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut de Lencastre Novaes Letícia Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut Mazzola Priscila Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas, Campinas, Brazil aut Penna Thereza Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut Júnior Adalberto Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/12(2013-12-01), 3456-3461 1935-5130 6:12<3456 2013 6 11947 https://doi.org/10.1007/s11947-012-1008-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-012-1008-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jozala Angela Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Lopes André Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- de Lencastre Novaes Letícia Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Mazzola Priscila Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas, Campinas, Brazil aut NATIONALLICENCE 700 1- Penna Thereza Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Júnior Adalberto Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/12(2013-12-01), 3456-3461 1935-5130 6:12<3456 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer