naa a22 4500 510725422 CHVBK 20180411082933.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11947-012-0998-z doi (NATIONALLICENCE)springer-10.1007/s11947-012-0998-z Recognizing Age at Slaughter of Cattle from Beef Samples Using GC/MS-SPME Chromatographic Method [Elektronische Daten] [Rocío Santander, Werner Creixell, Elizabeth Sánchez, Gerda Tomic, José Silva, Cristian Acevedo] Beef from cattle less than 30months old at the point of slaughter are generally sold in meat markets. Though the age of the animal can generally be estimated by dentition, this method cannot be applied to a carcass or a piece of meat from which the teeth have been removed. Since gas chromatography of volatile organic compounds has been used to classify biological samples, this technique was used for classifying meat obtained from cattle aged over 30months (OTM) at point of slaughter. Volatile organic compounds of fresh meat (M. longissimus dorsi) samples obtained from cattle of different age and dentition (two, four, and six teeth) at point of slaughter were analyzed using gas chromatography/mass spectrum (GC/MS). Six volatile organic compounds were selected (hexanoic acid, benzoic acid, octanoid acid, tridecane, tetradecane, and 4-formylbenzoic acid) and confirmed by using chemical standards and Kovat's indexes. The chromatogram data were used to train two classifiers, one based on the partial least square (PLS) method and another based on support vector machine (SVM), but only SVM showed high accuracy. Data exploration based on the principal component analysis allowed for comprehension of the data distribution, proposing a new version of PLS in a parallel configuration. This modified version of the PLS algorithm showed better accuracy than the SVM classifier. In conclusion, volatile organic compounds analyzed by GC/MS-solid-phase microextraction can be used to recognize OTM beef. Springer Science+Business Media New York, 2012 Beef nationallicence Gas chromatography nationallicence OTM nationallicence SPME nationallicence Volatile organic compounds nationallicence Santander Rocío Universidad de Santiago de Chile, Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Avenida Ecuador 3769, Estación Central, Santiago, Chile aut Creixell Werner Universidad Técnica Federico Santa María, Departamento de Electrónica, Avenida España 1680, Valparaíso, Chile aut Sánchez Elizabeth Universidad Técnica Federico Santa María, Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile aut Tomic Gerda Universidad de Santiago de Chile, Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Avenida Ecuador 3769, Estación Central, Santiago, Chile aut Silva José Universidad de Santiago de Chile, Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Avenida Ecuador 3769, Estación Central, Santiago, Chile aut Acevedo Cristian Universidad Técnica Federico Santa María, Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile aut Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/12(2013-12-01), 3345-3352 1935-5130 6:12<3345 2013 6 11947 https://doi.org/10.1007/s11947-012-0998-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-012-0998-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santander Rocío Universidad de Santiago de Chile, Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Avenida Ecuador 3769, Estación Central, Santiago, Chile aut NATIONALLICENCE 700 1- Creixell Werner Universidad Técnica Federico Santa María, Departamento de Electrónica, Avenida España 1680, Valparaíso, Chile aut NATIONALLICENCE 700 1- Sánchez Elizabeth Universidad Técnica Federico Santa María, Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile aut NATIONALLICENCE 700 1- Tomic Gerda Universidad de Santiago de Chile, Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Avenida Ecuador 3769, Estación Central, Santiago, Chile aut NATIONALLICENCE 700 1- Silva José Universidad de Santiago de Chile, Departamento de Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Avenida Ecuador 3769, Estación Central, Santiago, Chile aut NATIONALLICENCE 700 1- Acevedo Cristian Universidad Técnica Federico Santa María, Centro de Biotecnología, Avenida España 1680, Valparaíso, Chile aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/12(2013-12-01), 3345-3352 1935-5130 6:12<3345 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer