naa a22 4500 510725724 CHVBK 20180411082934.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s11947-011-0708-2 doi (NATIONALLICENCE)springer-10.1007/s11947-011-0708-2 Detection of Salmonella typhimurium Grown Directly on Tomato Surface Using Phage-Based Magnetoelastic Biosensors [Elektronische Daten] [Mi-Kyung Park, Suiqiong Li, Bryan Chin] A phage-based magnetoelastic (ME) biosensor method is being developed for on-site pathogen detection in fresh fruits and vegetables. Salmonella typhimurium was directly grown with minimal nutrients on tomato surfaces in order to mimic natural environmental conditions. S. typhimurium was inoculated on the surface of fresh tomatoes, and ME biosensors were used to detect the bacteria. The populations of S. typhimurium after 24-hincubation time at 37°C and 100% relative humidity were 6.1 and 7.8logCFU/cm2 after starting with initial inoculations of 3.0 and 5.0logCFU/cm2, respectively. After evident growth, measurement sensors with E2 phage immobilized on their surfaces and control sensors devoid of E2 phage were placed on the inoculated tomato surfaces and the resonant frequency shifts were measured. As the population of S. typhimurium increased, the resonant frequency shifts of the measurement sensors significantly increased (P < 0.01), exhibiting 6,680 ± 665 and 9,384 ± 457Hz for the populations of 6.1 and 7.8logCFU/cm2, respectively. SEM images confirmed that the measurement sensor resonant frequency shifts were due to S. typhimurium binding with E2 phage. This study demonstrated that the ME biosensor method could detect S. typhimurium grown directly on tomato surfaces with limited sample preparation procedures. Therefore, the ME biosensor could be applied as a cost and time effective, relatively simple, practically suitable on-site detection method for S. typhimurium in fresh produce. Springer Science+Business Media, LLC, 2011 Magnetoelastic biosensor nationallicence Salmonella typhimurium nationallicence Bacteriophage nationallicence Tomato nationallicence Spot inoculation nationallicence Park Mi-Kyung Materials Research and Education Center, Auburn University, 275 Wilmore Labs, AL 36849, Auburn, USA aut Li Suiqiong Materials Research and Education Center, Auburn University, 275 Wilmore Labs, AL 36849, Auburn, USA aut Chin Bryan Materials Research and Education Center, Auburn University, 275 Wilmore Labs, AL 36849, Auburn, USA aut Food and Bioprocess Technology Springer-Verlag 6/3(2013-03-01), 682-689 1935-5130 6:3<682 2013 6 11947 https://doi.org/10.1007/s11947-011-0708-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-011-0708-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Mi-Kyung Materials Research and Education Center, Auburn University, 275 Wilmore Labs, AL 36849, Auburn, USA aut NATIONALLICENCE 700 1- Li Suiqiong Materials Research and Education Center, Auburn University, 275 Wilmore Labs, AL 36849, Auburn, USA aut NATIONALLICENCE 700 1- Chin Bryan Materials Research and Education Center, Auburn University, 275 Wilmore Labs, AL 36849, Auburn, USA aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer-Verlag 6/3(2013-03-01), 682-689 1935-5130 6:3<682 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer