naa a22 4500 51072602X CHVBK 20180411082935.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11947-012-0956-9 doi (NATIONALLICENCE)springer-10.1007/s11947-012-0956-9 Pilot-Scale Gas Double-Dynamic Solid-State Fermentation for the Production of Industrial Enzymes [Elektronische Daten] [Qin He, Hongzhang Chen] Presently, the modern solid-state fermentation (SSF) industry remains traditional; there are few designs available for bioreactors operating in SSF on a large scale, which is mainly caused by the problems in engineering that appear when scaling up this technique. Gas double-dynamic solid-state fermentation (GDD-SSF) technology has been proven available in industrial production of biopesticide. However, there are few other examples using the GDD-SSF technology in other fermentation industries. To test its applicability in other fermentation industries, the self-made pilot-scale bioreactor for GDD-SSF of 800L was used for the production of various typical enzymes. The comparing effects of liquid-solid ratio and air pressure on enzyme production in different fermentation modes were investigated. Results showed that GDD-SSF offered greater advantages to the static SSF in industrial enzyme preparation, such as pectinase, glucoamylase, protease, and cellulase. It could not only improve enzyme activities with a largest increase reached 2.84 times but also shorten the fermentation period. Furthermore, decreased temperature gradient was found in GDD-SSF, which is crucial for online controlling in industry production. Thus, the necessary and successful pilot-scale fermentation of various enzymes was a rational indication of applicability to large-scale SSF by GDD-SSF. Springer Science+Business Media, LLC, 2012 Industrial enzymes nationallicence Enzyme activity nationallicence Gas double-dynamic solid-state fermentation (GDD-SSF) nationallicence Pilot scale nationallicence Solid-state fermentation nationallicence He Qin National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, People's Republic of China aut Chen Hongzhang National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, People's Republic of China aut Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/10(2013-10-01), 2916-2924 1935-5130 6:10<2916 2013 6 11947 https://doi.org/10.1007/s11947-012-0956-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-012-0956-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Qin National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Hongzhang National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/10(2013-10-01), 2916-2924 1935-5130 6:10<2916 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer