naa a22 4500 510726127 CHVBK 20180411082936.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11947-011-0741-1 doi (NATIONALLICENCE)springer-10.1007/s11947-011-0741-1 Inactivation of Foodborne Pathogens by NiO/TiO2 Composite Nanofibers: A Novel Biomaterial System [Elektronische Daten] [Touseef Amna, M. Hassan, Ayman Yousef, Amrita Mishra, Nasser Barakat, Myung-Seob Khil, Hak Kim] This study presents the fabrication and characterization of novel NiO/TiO2 composite nanofibers and their antibacterial activity. The utilized NiO/TiO2 composite nanofibers were prepared by electrospinning of a sol-gel composed of nickel nitrate hexahydrate, titanium isopropoxide and poly(vinyl acetate). The obtained electrospun nanofiberous mat was vacuum dried at 80°C and then calcined at 600°C in air for 2h. The physicochemical properties of the synthesized nanofibers were determined by X-ray diffraction pattern, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, electron probe microanalysis and transmission electron microscopy. The antibacterial activity was tested against four common foodborne pathogenic bacteria viz., Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Klebsiella pneumoniae by minimum inhibitory concentration (MIC) method taking five different concentrations (5-45μg/ml). Our investigation reveals that the lowest concentration of NiO/TiO2 composite solution inhibiting the growth of tested strains was found to be 5μg/ml. TEM analysis demonstrated that the exposure of the selected microbial strains to the composite nanofibers led to disruption of cell membranes and depressed the activity of some membranous enzymes, which caused bacteria to die eventually. Furthermore, the results illustrate that the combination of NiO and TiO2 can be synergistic and resulted in superior antimicrobial activity of NiO/TiO2 composite nanofibers. To sum up, novel NiO/TiO2 composite nanofibers that possess large surface-to-volume ratio with excellent antimicrobial activity were fabricated that can be used to inhibit the microbial growth associated with food stuff. Springer Science+Business Media, LLC, 2011 Electrospinning nationallicence NiO/TiO2 composite nanofibers nationallicence Antibacterial mechanism nationallicence MIC foodborne pathogens nationallicence Amna Touseef Center for Healthcare Technology Development, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut Hassan M. Department of Organic Materials and Fiber Engineering, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut Yousef Ayman Center for Healthcare Technology Development, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut Mishra Amrita Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut Barakat Nasser Department of Organic Materials and Fiber Engineering, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut Khil Myung-Seob Department of Organic Materials and Fiber Engineering, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut Kim Hak Center for Healthcare Technology Development, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut Food and Bioprocess Technology Springer-Verlag 6/4(2013-04-01), 988-996 1935-5130 6:4<988 2013 6 11947 https://doi.org/10.1007/s11947-011-0741-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-011-0741-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Amna Touseef Center for Healthcare Technology Development, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut NATIONALLICENCE 700 1- Hassan M. Department of Organic Materials and Fiber Engineering, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut NATIONALLICENCE 700 1- Yousef Ayman Center for Healthcare Technology Development, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut NATIONALLICENCE 700 1- Mishra Amrita Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut NATIONALLICENCE 700 1- Barakat Nasser Department of Organic Materials and Fiber Engineering, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut NATIONALLICENCE 700 1- Khil Myung-Seob Department of Organic Materials and Fiber Engineering, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut NATIONALLICENCE 700 1- Kim Hak Center for Healthcare Technology Development, Chonbuk National University, 561-756, Jeonju, Republic of Korea aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer-Verlag 6/4(2013-04-01), 988-996 1935-5130 6:4<988 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer