naa a22 4500 51072650X CHVBK 20180411082937.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11947-012-1018-z doi (NATIONALLICENCE)springer-10.1007/s11947-012-1018-z Improvement of Freeze-Dried Lactobacillus Plantarum Survival Using Water Extracts and Crude Fibers from Food Crops [Elektronische Daten] [Tipparat Hongpattarakere, Patcharawan Rattanaubon, Nirunya Buntin] A synbiotic product of combined Lactobacillus plantarum TISTR 875 with water extracts and crude fibers from corn, mungbean, and soybean was formulated to investigate the survival of L. plantarum during freeze-drying and storage. The impacts of those water extracts and crude fibers on probiotic survival were determined in both a cultural medium and a freeze-drying medium. L. plantarum cultivated in de Man, Rogosa, and Sharpe (MRS) broth containing 2% of water extract from soybean with 2% mungbean fiber showed only 0.11log CFU/ml cell reduction. The survival of L. plantarum harvested at the late log phase, mid stationary, phase and late stationary phase did not show statistical significance (P > 0.05), whereas an initial pH of 6.5 and growth temperature of 37°C showed greater impact (P < 0.05). The addition of corn extract to the freeze-drying medium as a cryoprotectant had a similar effect on L. plantarum survival as sucrose, but it was better (P < 0.05) than fructo-oligosaccharide and exopolysaccharides from Weissella cibaria A2, soybean extract, mungbean extract, soybean, corn, and mungbean fibers. A protective coating of corn extract was revealed and observed using scanning electron microscopy. The freeze-dried L. plantarum, cultivated in MRS broth containing soybean extract and mungbean fiber with corn extract as a cryoprotectant, retained high viability of 7.21 and 6.88log CFU/ml after 8-week storage in a vacuum-packed aluminum foil-laminated polyethylene sachet and a nitrogen-flushed glass vial, respectively. Springer Science+Business Media New York, 2012 Freeze-drying nationallicence Probiotic nationallicence Synbiotics nationallicence Mungbean nationallicence Corn nationallicence Exopolysaccharides nationallicence L . plantarum nationallicence Hongpattarakere Tipparat Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, 90112, Hat Yai, Songkhla, Thailand aut Rattanaubon Patcharawan Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, 90112, Hat Yai, Songkhla, Thailand aut Buntin Nirunya Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, 90112, Hat Yai, Songkhla, Thailand aut Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/8(2013-08-01), 1885-1896 1935-5130 6:8<1885 2013 6 11947 https://doi.org/10.1007/s11947-012-1018-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-012-1018-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hongpattarakere Tipparat Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, 90112, Hat Yai, Songkhla, Thailand aut NATIONALLICENCE 700 1- Rattanaubon Patcharawan Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, 90112, Hat Yai, Songkhla, Thailand aut NATIONALLICENCE 700 1- Buntin Nirunya Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, 90112, Hat Yai, Songkhla, Thailand aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/8(2013-08-01), 1885-1896 1935-5130 6:8<1885 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer