naa a22 4500 510726941 CHVBK 20180411082939.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11947-011-0666-8 doi (NATIONALLICENCE)springer-10.1007/s11947-011-0666-8 Effect of Pulsed Ultraviolet Light and High Hydrostatic Pressure on the Antigenicity of Almond Protein Extracts [Elektronische Daten] [Yiqiao Li, Wade Yang, Si-Yin Chung, Haiqiang Chen, Mu Ye, Arthur Teixeira, Jesse Gregory, Bruce Welt, Sandra Shriver] The efficacy of pulsed ultraviolet light (PUV) and high hydrostatic pressure (HHP) on the IgE binding to the almond extracts was studied using sodium dodecyl sulfate polyacrylamide-gel electrophoresis, Western blot, and enzyme-linked immunosorbent assay (ELISA) probed with human plasma containing IgE antibodies to almond allergens and a polyclonal antibody against almond major protein. Crude almond protein extracts were treated with PUV (3pulses/s, 10cm from lamp) for 0.5, 1, 2, 3, 4, 6, 7, and 10min. In comparison, boiling treatments were also carried out. The HHP treatments were conducted at 600MPa for 5, 15, and 30min at three temperatures of 4°C, 21°C, and 70°C. Western blots and indirect ELISA demonstrated a reduction in the levels of allergens and IgE binding in PUV-treated extracts at 7min, which was found to be the optimal time for PUV exposure. Boiling was not as effective as PUV in reducing the overall IgE-binding of the almond extracts. Unlike PUV, HHP did not affect the allergen levels and IgE binding under the conditions tested. Springer Science+Business Media, LLC, 2011 Almond nationallicence Allergen nationallicence Pulsed UV light nationallicence High pressure nationallicence IgE binding nationallicence Li Yiqiao Department of Agriculture and Biological Engineering, University of Florida, 207 Frazier Rogers Hall, 32611, Gainesville, FL, USA aut Yang Wade Department of Food Science and Human Nutrition, University of Florida, 359 FSHN Bldg. Newell Drive, 32611, Gainesville, FL, USA aut Chung Si-Yin USDA-ARS Southern Regional Research Center, 1100 Robert E. Lee Blvd., 70124, New Orleans, LA, USA aut Chen Haiqiang Department of Animal and Food Sciences, University of Delaware, 020 Townsend Hall, 19716, Newark, DE, USA aut Ye Mu Department of Animal and Food Sciences, University of Delaware, 020 Townsend Hall, 19716, Newark, DE, USA aut Teixeira Arthur Department of Agriculture and Biological Engineering, University of Florida, 207 Frazier Rogers Hall, 32611, Gainesville, FL, USA aut Gregory Jesse Department of Food Science and Human Nutrition, University of Florida, 359 FSHN Bldg. Newell Drive, 32611, Gainesville, FL, USA aut Welt Bruce Department of Agriculture and Biological Engineering, University of Florida, 207 Frazier Rogers Hall, 32611, Gainesville, FL, USA aut Shriver Sandra Department of Food Science and Human Nutrition, University of Florida, 359 FSHN Bldg. Newell Drive, 32611, Gainesville, FL, USA aut Food and Bioprocess Technology Springer-Verlag 6/2(2013-02-01), 431-440 1935-5130 6:2<431 2013 6 11947 https://doi.org/10.1007/s11947-011-0666-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-011-0666-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Yiqiao Department of Agriculture and Biological Engineering, University of Florida, 207 Frazier Rogers Hall, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Yang Wade Department of Food Science and Human Nutrition, University of Florida, 359 FSHN Bldg. Newell Drive, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Chung Si-Yin USDA-ARS Southern Regional Research Center, 1100 Robert E. Lee Blvd., 70124, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Chen Haiqiang Department of Animal and Food Sciences, University of Delaware, 020 Townsend Hall, 19716, Newark, DE, USA aut NATIONALLICENCE 700 1- Ye Mu Department of Animal and Food Sciences, University of Delaware, 020 Townsend Hall, 19716, Newark, DE, USA aut NATIONALLICENCE 700 1- Teixeira Arthur Department of Agriculture and Biological Engineering, University of Florida, 207 Frazier Rogers Hall, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Gregory Jesse Department of Food Science and Human Nutrition, University of Florida, 359 FSHN Bldg. Newell Drive, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Welt Bruce Department of Agriculture and Biological Engineering, University of Florida, 207 Frazier Rogers Hall, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Shriver Sandra Department of Food Science and Human Nutrition, University of Florida, 359 FSHN Bldg. Newell Drive, 32611, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer-Verlag 6/2(2013-02-01), 431-440 1935-5130 6:2<431 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer