naa a22 4500 510727077 CHVBK 20180411082939.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11947-011-0703-7 doi (NATIONALLICENCE)springer-10.1007/s11947-011-0703-7 Impact of Electric Pulse Treatment on Selective Extraction of Intracellular Compounds from Saccharomyces cerevisiae Yeasts [Elektronische Daten] [Dan Liu, N. Lebovka, E. Vorobiev] Treatments by high-voltage electrical discharges (HVED, needle-plate electrode geometry, U = 40kV, t p ≈ 0.5μs) and pulsed electric field (PEF, plate-plate electrode geometry, E = 5-40kV/cm, t p ≈ 8.3μs) were evaluated as tools for selective extraction of different intracellular components from the wine Saccharomyces cerevisiae (bayanus) yeasts in a 0.5% (w/w) aqueous suspension. The pulses in the form of damped oscillations and exponential decay were applied in HVED and PEF modes of treatment, respectively. The extraction efficiency results obtained using HVED and PEF techniques were compared with those for high-pressure homogenization technique. The HVED and PEF treatments always resulted in incomplete damage of yeast cells, though efficiency of HVED was higher than that of PEF. The high selectivity of extraction of ionic substances, proteins, and nucleic acids was demonstrated; e.g., electric pulse treatments at E = 40kV/cm and N = 500 allowed extraction of ≈80% and ≈70% of ionic substances, ≈4% and ≈1% of proteins and ≈30% and ≈16% of nucleic acids in cases of HVED and PEF modes, respectively. Springer Science+Business Media, LLC, 2011 Electroporation nationallicence Pulsed electric fields nationallicence High voltage electrical discharges nationallicence Selective extraction nationallicence Saccharomyces cerevisiae yeast nationallicence Liu Dan Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable, Centre de Recherche de Royallieu, Université de Technologie de Compiègne, B.P. 20529-60205, Compiègne Cedex, France aut Lebovka N. Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable, Centre de Recherche de Royallieu, Université de Technologie de Compiègne, B.P. 20529-60205, Compiègne Cedex, France aut Vorobiev E. Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable, Centre de Recherche de Royallieu, Université de Technologie de Compiègne, B.P. 20529-60205, Compiègne Cedex, France aut Food and Bioprocess Technology Springer-Verlag 6/2(2013-02-01), 576-584 1935-5130 6:2<576 2013 6 11947 https://doi.org/10.1007/s11947-011-0703-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-011-0703-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Dan Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable, Centre de Recherche de Royallieu, Université de Technologie de Compiègne, B.P. 20529-60205, Compiègne Cedex, France aut NATIONALLICENCE 700 1- Lebovka N. Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable, Centre de Recherche de Royallieu, Université de Technologie de Compiègne, B.P. 20529-60205, Compiègne Cedex, France aut NATIONALLICENCE 700 1- Vorobiev E. Département de Génie des procédés industriels, Unité Transformations Intégrées de la Matière Renouvelable, Centre de Recherche de Royallieu, Université de Technologie de Compiègne, B.P. 20529-60205, Compiègne Cedex, France aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer-Verlag 6/2(2013-02-01), 576-584 1935-5130 6:2<576 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer