naa a22 4500 510727506 CHVBK 20180411082941.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s11947-012-0937-z doi (NATIONALLICENCE)springer-10.1007/s11947-012-0937-z Pasteurization of Apple Juice Contaminated with Escherichia coli by a Combined UV-Mild Temperature Treatment [Elektronische Daten] [Elisa Gayán, María Serrano, Silvia Monfort, Ignacio Álvarez, Santiago Condón] The bactericidal efficacy of ultraviolet (UV) treatments to fruit juices is limited because of their low UV transmittance; therefore, it is necessary to design combined processes to improve their lethality. This investigation was carried out to determinate the lethal effect of UV-C treatments at mild temperatures (UV-H treatments) on the UV-resistant Escherichia coli strain Spanish Type Culture Collection (STCC) 4201 suspended in apple juice. A synergistic effect was observed and the optimum temperature for the combined process was established. Subsequently, the effect of the optimized treatment on the lethality of an E. coli cocktail (STCC 4201, STCC 471, American Type Culture Collection (ATCC) 27325, ATCC 25922, and O157:H7 Chapman strain) and on freshly squeezed apple juice quality was evaluated. A UV treatment of 20.33J/mL reached 0.61 ± 0.01, 0.83 ± 0.07, 1.38 ± 0.04, 1.97 ± 0.06, 3.72 ± 0.14, 5.67 ± 0.61, and more than 6 log10 cycles of inactivation at 25.0, 40.0, 50.0, 52.5, 55.0, 57.5, and 60.0°C, respectively. The optimum conditions for exploiting the synergistic effects were UV doses of 27.10J/mL, temperature of 55.0°C, and 3.58min of treatment time. This treatment guaranteed more of 5 log10 reductions of the cocktail of five strains of E. coli without affecting pH, °Brix, and acidity of freshly squeezed apple juice. The UV-H treatment did not increase the loss of ascorbic acid compared to the same UV treatment at room temperature but approximately doubled the inactivation of polifenoloxidase. Springer Science+Business Media, LLC, 2012 Pasteurization nationallicence Apple juice nationallicence Escherichia coli nationallicence Ultraviolet irradiation nationallicence Heat treatments nationallicence Gayán Elisa Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut Serrano María Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut Monfort Silvia Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut Álvarez Ignacio Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut Condón Santiago Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/11(2013-11-01), 3006-3016 1935-5130 6:11<3006 2013 6 11947 https://doi.org/10.1007/s11947-012-0937-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11947-012-0937-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gayán Elisa Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut NATIONALLICENCE 700 1- Serrano María Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut NATIONALLICENCE 700 1- Monfort Silvia Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut NATIONALLICENCE 700 1- Álvarez Ignacio Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut NATIONALLICENCE 700 1- Condón Santiago Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain aut NATIONALLICENCE 773 0- Food and Bioprocess Technology Springer US; http://www.springer-ny.com 6/11(2013-11-01), 3006-3016 1935-5130 6:11<3006 2013 6 11947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer