naa a22 4500 510727921 CHVBK 20180411082942.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s11748-013-0224-6 doi (NATIONALLICENCE)springer-10.1007/s11748-013-0224-6 Current status and perspective of EBUS-TBNA [Elektronische Daten] [Takahiro Nakajima, Kazuhiro Yasufuku, Ichiro Yoshino] In 2002, a new bronchoscope equipped with a convex type ultrasound probe on the tip was introduced into clinical practice. This convex probe endobronchial ultrasound (CP-EBUS) provides a long axis image of surrounding structures of the major airway. The CP-EBUS combined with a dedicated biopsy needle allows real-time endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) of mediastinal and hilar lymph nodes. Samples obtained from EBUS-TBNA can be used for pathological diagnosis including immunohistochemistry. EBUS-TBNA is now being performed worldwide for several indications including mediastinal staging, mediastinal pathology and for benign diseases. For mediastinal staging in lung cancer, the diagnostic yield of EBUS-TBNA is comparable to surgical staging in patients with enlarged lymph nodes. In addition, EBUS-TBNA can be used for molecular analysis of tumor cells obtained during the procedure. DNA mutation testing using EBUS-TBNA samples was first reported in 2007 for a chondrosarcoma case. Also, isolated DNA from EBUS-TBNA samples was applied for epidermal growth factor receptor mutation testing and aberrant methylation analysis in patients with lung cancer. RNA was also isolated from properly stored EBUS-TBNA samples and used for gene expression research and aberrant fusion gene detection in lung cancer patients. Optimization of tissue banking methods of EBUS-TBNA specimens was also investigated. Currently, EBUS-TBNA samples can be used for comprehensive mRNA and miRNA expression analysis. EBUS-TBNA will become an important modality for bronchoscopists in the era of personalized medicine in lung cancer. The Japanese Association for Thoracic Surgery, 2013 Endobronchial ultrasound nationallicence Transbronchial biopsy nationallicence Lung cancer nationallicence Biomarker nationallicence Translational research nationallicence Nakajima Takahiro Department of General Thoracic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Japan aut Yasufuku Kazuhiro Division of Thoracic Surgery, Toronto General Hospital, University Health Network, University of Toronto, 200 Elizabeth street, Toronto, Ontario, Canada aut Yoshino Ichiro Department of General Thoracic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Japan aut General Thoracic and Cardiovascular Surgery Springer Japan 61/7(2013-07-01), 390-396 1863-6705 61:7<390 2013 61 11748 https://doi.org/10.1007/s11748-013-0224-6 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11748-013-0224-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakajima Takahiro Department of General Thoracic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Japan aut NATIONALLICENCE 700 1- Yasufuku Kazuhiro Division of Thoracic Surgery, Toronto General Hospital, University Health Network, University of Toronto, 200 Elizabeth street, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Yoshino Ichiro Department of General Thoracic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Japan aut NATIONALLICENCE 773 0- General Thoracic and Cardiovascular Surgery Springer Japan 61/7(2013-07-01), 390-396 1863-6705 61:7<390 2013 61 11748 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer