naa a22 4500 510730124 CHVBK 20180411082949.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11295-012-0539-y doi (NATIONALLICENCE)springer-10.1007/s11295-012-0539-y Identification, characterization and mapping of EST-derived SSRs from the cacao- Ceratocystis cacaofunesta interaction [Elektronische Daten] [Rogério Santos, Didier Clement, Lívia Lemos, Thierry Legravre, Claire Lanaud, Raymond Schnell, Jose Pires, Uilson Lopes, Fabienne Micheli, Karina Gramacho] Ceratocystis cacaofunesta is an ascomycete responsible for the lethal wilt disease of cacao (Theobroma cacao L.). Marker-assisted selection combined with conventional breeding is one powerful approach to improve cacao resistance to Ceratocystis wilt. In this study we screened a set of ESTs obtained from cacao elicited with C. cacaofunesta to identify EST-SSRs and test their efficacy for mapping. Among the 3,432 ESTs analysed, 384 contained SSRs and 428 EST-SSRs were identified, mainly dinucleotides (78.5%), with four repeats (75.23%), and preferentially AG/CT motif (25.47%). Gene ontology function was assigned to the ESTs containing SSRs: 4.04% belonged to "defense response” category, with 20.69% of them to the sub-category "defense response to fungi”. In relation to the ORF, the same quantity of EST-SSRs was observed in the 5′ UTR, ORF and the 3′ UTR (about 30%). From the 428 EST-SSRs identified, 12 were polymorphic, revealing a total of 41 alleles. The number of alleles per locus ranged from 2 to 6, with an average of 3.41. Four EST-SSRs were mapped on the F2 Sca 6 × ICS 1 population segregating for Ceratocystis wilt, and were distributed on the 2, 3, 4 and 8 linkage groups. These markers will have potential applications in linkage mapping and will be valuable for the research community to improve the cacao breeding program. Springer-Verlag, 2012 Ceratocystis wilt nationallicence Molecular breeding nationallicence Resistance nationallicence AFLP : Amplified fragment length polymorphism nationallicence EST : Expressed sequence tag nationallicence GO : Gene ontology nationallicence HWE : Hardy-Weinberg equilibrium nationallicence ICS 1 : Imperial College Selections 1 nationallicence LG : Linkage group nationallicence MAS : Marker-assisted selection nationallicence ORF : Open reading frame nationallicence PIC : Polymorphic information content nationallicence RAPD : Random amplified polymorphic DNA nationallicence RFLP : Restriction fragment length polymorphism nationallicence Sca 6 : Scavina 6 nationallicence SSH : Suppressive and subtractive hybridization nationallicence SNP : Single-nucleotide polymorphism nationallicence SSR : Simple sequence repeats nationallicence UTR : Untranslated region nationallicence Santos Rogério Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut Clement Didier Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut Lemos Lívia Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut Legravre Thierry CIRAD, UMR AGAP, Avenue Agropolis TA96/03, 34398, Montpellier cedex 5, France aut Lanaud Claire CIRAD, UMR AGAP, Avenue Agropolis TA96/03, 34398, Montpellier cedex 5, France aut Schnell Raymond USDA-ARS, 13601 Old Cutler Rd, 33158-0000, Miami, FL, USA aut Pires Jose Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut Lopes Uilson Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut Micheli Fabienne CIRAD, UMR AGAP, Avenue Agropolis TA96/03, 34398, Montpellier cedex 5, France aut Gramacho Karina Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 117-127 1614-2942 9:1<117 2013 9 11295 https://doi.org/10.1007/s11295-012-0539-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0539-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santos Rogério Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut NATIONALLICENCE 700 1- Clement Didier Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut NATIONALLICENCE 700 1- Lemos Lívia Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut NATIONALLICENCE 700 1- Legravre Thierry CIRAD, UMR AGAP, Avenue Agropolis TA96/03, 34398, Montpellier cedex 5, France aut NATIONALLICENCE 700 1- Lanaud Claire CIRAD, UMR AGAP, Avenue Agropolis TA96/03, 34398, Montpellier cedex 5, France aut NATIONALLICENCE 700 1- Schnell Raymond USDA-ARS, 13601 Old Cutler Rd, 33158-0000, Miami, FL, USA aut NATIONALLICENCE 700 1- Pires Jose Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut NATIONALLICENCE 700 1- Lopes Uilson Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut NATIONALLICENCE 700 1- Micheli Fabienne CIRAD, UMR AGAP, Avenue Agropolis TA96/03, 34398, Montpellier cedex 5, France aut NATIONALLICENCE 700 1- Gramacho Karina Cocoa Research Center, CEPLAC/CEPEC, 45600-970, Itabuna, Bahia, Brazil aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 117-127 1614-2942 9:1<117 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer