naa a22 4500 51073023X CHVBK 20180411082950.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11295-012-0536-1 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0536-1 Molecular cloning and characterization of flavonol synthase in Acacia confusa [Elektronische Daten] [Hwei-Choong Toh, Sheng-Yang Wang, Shang-Tzen Chang, Fang-Hua Chu] Flavonoids are abundant in the leaves, flowers, branches, bark, and heartwood of Acacia confusa Merr. (Leguminosae), and the extracts have been found to have strong antioxidant activities. Flavonols are the major class of flavonoids found in A. confusa. The full-length gene (designated as AcFLS) contains an open reading frame of 996 nucleotides encoding 331 amino acid residues with a predicted molecular weight of 38kDa and pI of 5.7. AcFLS mRNAs were abundant in the seedlings leaves and the flowers. Wounding by cutting leaves induced maximum AcFLS mRNA accumulation 6-12h after treatment. AcFLS could enzymatically transform dihydromyricetin, dihydroquercetin, and naringenin into the flavonols myricetin, quercetin, and kaempferol, respectively. AcFLS could bind these three substrates with similar affinity. Springer-Verlag, 2012 Acacia confusa nationallicence Biosynthesis nationallicence Flavonoids nationallicence Flavonols nationallicence Kaempferol nationallicence Myricetin nationallicence Quercetin nationallicence Protein structure modeling nationallicence Toh Hwei-Choong School of Forestry and Resource Conservation, National Taiwan University, 106, Taipei, Taiwan aut Wang Sheng-Yang Department of Forestry, National Chung-Hsing University, 402, Taichung, Taiwan aut Chang Shang-Tzen School of Forestry and Resource Conservation, National Taiwan University, 106, Taipei, Taiwan aut Chu Fang-Hua School of Forestry and Resource Conservation, National Taiwan University, 106, Taipei, Taiwan aut Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 85-92 1614-2942 9:1<85 2013 9 11295 https://doi.org/10.1007/s11295-012-0536-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0536-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toh Hwei-Choong School of Forestry and Resource Conservation, National Taiwan University, 106, Taipei, Taiwan aut NATIONALLICENCE 700 1- Wang Sheng-Yang Department of Forestry, National Chung-Hsing University, 402, Taichung, Taiwan aut NATIONALLICENCE 700 1- Chang Shang-Tzen School of Forestry and Resource Conservation, National Taiwan University, 106, Taipei, Taiwan aut NATIONALLICENCE 700 1- Chu Fang-Hua School of Forestry and Resource Conservation, National Taiwan University, 106, Taipei, Taiwan aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 85-92 1614-2942 9:1<85 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer