naa a22 4500 510730248 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11295-012-0542-3 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0542-3 High-throughput genotyping in citrus accessions using an SNP genotyping array [Elektronische Daten] [Hiroshi Fujii, Takehiko Shimada, Keisuke Nonaka, Masayuki Kita, Takeshi Kuniga, Tomoko Endo, Yoshinori Ikoma, Mitsuo Omura] We developed a 384 multiplexed SNP array, named CitSGA-1, for the genotyping of Citrus cultivars, and evaluated the performance and reliability of the genotyping. SNPs were surveyed by direct sequence comparison of the sequence tagged site (STS) fragment amplified from genomic DNA of cultivars representing the genetic diversity of citrus breeding in Japan. Among 1497 SNPs candidates, 384 SNPs for a high-throughput genotyping array were selected based on physical parameters of Illumina's bead array criteria. The assay using CitSGA-1 was applied to a hybrid population of 88 progeny and 103 citrus accessions for breeding in Japan, which resulted in 73,726 SNP calls. A total of 351 SNPs (91%) could call different genotypes among the DNA samples, resulting in a success rate for the assay comparable to previously reported rates for other plant species. To confirm the reliability of SNP genotype calls, parentage analysis was applied, and it indicated that the number of reliable SNPs and corresponding STSs were 276 and 213, respectively. The multiplexed SNP genotyping array reported here will be useful for the efficient construction of linkage map, for the detection of markers for marker-assisted breeding, and for the identification of cultivars. Springer-Verlag, 2012 High throughput nationallicence SNP nationallicence Genotyping nationallicence Citrus nationallicence Parentage nationallicence Fujii Hiroshi Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut Shimada Takehiko Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut Nonaka Keisuke Kuchinotsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 859-2501, Minamishimabara, Nagasaki, Japan aut Kita Masayuki Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut Kuniga Takeshi NARO Agricultural Research Center for Western Region, National Agriculture and Food Research Organization, 765-0053, Zentsuuji, Kagawa, Japan aut Endo Tomoko Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut Ikoma Yoshinori Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut Omura Mitsuo Faculty of Agriculture, Shizuoka University, 422-8529, Suruga, Shizuoka, Japan aut Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 145-153 1614-2942 9:1<145 2013 9 11295 https://doi.org/10.1007/s11295-012-0542-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0542-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujii Hiroshi Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Shimada Takehiko Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Nonaka Keisuke Kuchinotsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 859-2501, Minamishimabara, Nagasaki, Japan aut NATIONALLICENCE 700 1- Kita Masayuki Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Kuniga Takeshi NARO Agricultural Research Center for Western Region, National Agriculture and Food Research Organization, 765-0053, Zentsuuji, Kagawa, Japan aut NATIONALLICENCE 700 1- Endo Tomoko Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Ikoma Yoshinori Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 424-0292, Shimizu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Omura Mitsuo Faculty of Agriculture, Shizuoka University, 422-8529, Suruga, Shizuoka, Japan aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 145-153 1614-2942 9:1<145 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer