naa a22 4500 510730256 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11295-012-0534-3 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0534-3 Identification of differentially expressed genes related to coloration in red/green mutant pear ( Pyrus communis L.) [Elektronische Daten] [Jun Wu, Guang Zhao, Ya-Nan Yang, Wen-Quan Le, Muhammad Khan, Shao-Ling Zhang, Chao Gu, Wen-Jiang Huang] Fruit skin color is an important parameter of outer quality and plays an important role in attracting customers. In many plants, it is the result of coordinative regulation of anthocyanin pathway genes. In our study, the differential expression of cDNA library in a pair of pear mutant with red and green color was investigated to find candidate genes which might regulate the anthocyanin biosynthesis and control the coloration of pear. We constructed a cDNA library using the cDNA-amplified fragment length polymorphism approach to analyze the transcriptional differences between the original cultivar "Early red Doyenne du Comice” with high anthocyanin content in the peel and its green color mutant with comparatively low anthocyanin content. Altogether, 47 transcript-derived fragments, putatively involved in anthocyanin biosynthesis, primary metabolism, stress, and defense responses, were identified. The relationships of differentially expressed genes and coloration were investigated by quantitative real-time PCR with fruit skin samples at different developmental stages. A gene putatively involved in anthocyanin biosynthesis was found and named as PyMADS18. Its sequence is similar to genes reported in the literature as regulators of anthocyanin biosynthesis. The expression results indicate that PyMADS18 is likely to be involved in anthocyanin accumulation and regulation of anthocyanin synthesis in early fruit development of pear. Springer-Verlag, 2012 Pyrus communis L nationallicence Peel color nationallicence Anthocyanin nationallicence cDNA-AFLP nationallicence PyMADS18 nationallicence cDNA-AFLP : cDNA-Amplified Fragment Length Polymorphism nationallicence TDFs : Transcript Derived Fragments nationallicence CHS : Chalcone synthase nationallicence F3H : Flavonone 3-hydroxylase nationallicence CHI : Chalcone isomerase nationallicence DFR : Dihydroflavonol 4-reductase nationallicence ANS : Anthocyanin synthase nationallicence UFGT : UDP-glucose and flavonoid 3-O-glucosyl transferase nationallicence DAFB : Days After Full Blooming nationallicence qRT-PCR : Quantitative Reverse Transcription-Polymerase Chain Reaction nationallicence Wu Jun Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Zhao Guang Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Yang Ya-Nan Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Le Wen-Quan Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, 066600, Changli, Hebei, People's Republic of China aut Khan Muhammad Department of Natural Resources and Environmental Sciences, University of Illinois, 1201 W. Gregory, 61801, Urbana, IL, USA aut Zhang Shao-Ling Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Gu Chao Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Huang Wen-Jiang Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 75-83 1614-2942 9:1<75 2013 9 11295 https://doi.org/10.1007/s11295-012-0534-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0534-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Jun Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Guang Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Ya-Nan Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Le Wen-Quan Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, 066600, Changli, Hebei, People's Republic of China aut NATIONALLICENCE 700 1- Khan Muhammad Department of Natural Resources and Environmental Sciences, University of Illinois, 1201 W. Gregory, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Zhang Shao-Ling Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Gu Chao Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Huang Wen-Jiang Centre of Pear Engineering Technology Research, Nanjing Agricultural University, No. 1 Weigang Rd., 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 75-83 1614-2942 9:1<75 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer