naa a22 4500 510730272 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11295-012-0554-z doi (NATIONALLICENCE)springer-10.1007/s11295-012-0554-z DNA marker-assisted evaluation of fruit firmness at harvest and post-harvest fruit softening in a diverse apple germplasm [Elektronische Daten] [Hilde Nybom, Masoud Ahmadi-Afzadi, Jasna Sehic, Maarten Hertog] Several different genes have been proposed as responsible for fruit texture variability at harvest and/or after storage. We have analysed 127 apple cultivars for allelic composition in two key genes that are directly involved in the ethylene biosynthesis pathway, Md-ACS1 and Md-ACO1, and two other genes that are involved in cell wall degradation, Md-Exp7 and Md-PG1. Firmness was measured with a penetrometer at harvest and after 6 or 12weeks (early- and late-maturing cultivars, respectively) of cold storage. Maturation time was positively correlated with firmness at harvest and negatively correlated with fruit softening rate (difference between firmness at harvest and after storage, divided by number of weeks in storage). Polyploid cultivars showed significantly higher firmness at harvest compared to diploids. Alleles previously described as responsible for good texture were associated with significantly lower softening for Md-ACS1 and Md-PG1, but the opposite was noted for Md-EXP7. Results were nonsignificant for Md-ACO1. Allele frequencies were very uneven in all four loci, with the three most common multi-locus configurations accounting for 64% of the entire material. The predictive power of these genes was calculated with a partial least squares discriminant analysis, and these accounted for 15% of the observed variation in initial firmness and 18% for softening rate. Inclusion of maturation time, storage time (i.e. 6 or 12weeks) and initial firmness into the model however increased the predictability of softening rate to 38%. Dividing the material in modern (released after 1960) and old cultivars did not change the outcome of our analyses. Springer-Verlag, 2012 DNA marker nationallicence Fruit texture nationallicence Malus × domestica nationallicence Md-ACO1 nationallicence Md-ACS1 nationallicence Md-EXP7 nationallicence Md-PG1 nationallicence Nybom Hilde Balsgård, Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Fjälkestadsvägen 459, 29194, Kristianstad, Sweden aut Ahmadi-Afzadi Masoud Balsgård, Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Fjälkestadsvägen 459, 29194, Kristianstad, Sweden aut Sehic Jasna Balsgård, Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Fjälkestadsvägen 459, 29194, Kristianstad, Sweden aut Hertog Maarten BIOSYST-MeBioS, Katholieke Universiteit Leuven, Willem de Croylaan 42, bus 2428, 3001, Heverlee, Belgium aut Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 279-290 1614-2942 9:1<279 2013 9 11295 https://doi.org/10.1007/s11295-012-0554-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0554-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nybom Hilde Balsgård, Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Fjälkestadsvägen 459, 29194, Kristianstad, Sweden aut NATIONALLICENCE 700 1- Ahmadi-Afzadi Masoud Balsgård, Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Fjälkestadsvägen 459, 29194, Kristianstad, Sweden aut NATIONALLICENCE 700 1- Sehic Jasna Balsgård, Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Fjälkestadsvägen 459, 29194, Kristianstad, Sweden aut NATIONALLICENCE 700 1- Hertog Maarten BIOSYST-MeBioS, Katholieke Universiteit Leuven, Willem de Croylaan 42, bus 2428, 3001, Heverlee, Belgium aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 279-290 1614-2942 9:1<279 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer