naa a22 4500 510730302 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11295-012-0551-2 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0551-2 Genome-wide analysis of the LEA (late embryogenesis abundant) protein gene family in Populus trichocarpa [Elektronische Daten] [Ting Lan, Jie Gao, Qing-Yin Zeng] Late embryogenesis abundant (LEA) proteins are a large and highly diverse group of polypeptides that are believed to function in desiccation and freezing tolerance in plants. This report presents a genome-wide analysis of LEA proteins and their encoding genes in Populus trichocarpa. Fifty-three LEA genes were identified from the Populus genome and divided into eight groups. The LEA4 and LEA5 groups were found in green algae and all land plants, whereas the other six groups existed only in land plants, indicating that the LEA family underwent rapid expansion during the early evolution of land plants. A majority of Populus LEA proteins contained repeated motifs that were often specific to a LEA group. Except for PtLEA2-1 and PtLEA2-3, all Populus LEA proteins were highly hydrophilic. Examination of the chromosomal locations of Populus LEA genes revealed that 30% were arranged in tandem repeats, indicating that tandem duplications significantly contributed to the expansion of this gene family in Populus. Expression patterns of all Populus LEA genes under normal growth conditions and abiotic stress (salinity and drought) were investigated by reverse transcription polymerase chain reaction. Twelve of 53 Populus LEA genes were selectively expressed in a specific tissue and/or in response to a specific treatment. LEA genes also showed extensive divergence in expression patterns, even among those from the same group or gene cluster. The expression profiles revealed that the Populus LEA gene family was not involved systematically in the same regulatory pathway. Springer-Verlag, 2012 Late embryogenesis abundant nationallicence Gene family nationallicence Abiotic stresses nationallicence Gene expression nationallicence Lan Ting State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Gao Jie State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Zeng Qing-Yin State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 253-264 1614-2942 9:1<253 2013 9 11295 https://doi.org/10.1007/s11295-012-0551-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0551-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lan Ting State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Gao Jie State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 700 1- Zeng Qing-Yin State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 253-264 1614-2942 9:1<253 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer