naa a22 4500 510730353 CHVBK 20180411082950.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11295-012-0550-3 doi (NATIONALLICENCE)springer-10.1007/s11295-012-0550-3 A candidate gene for fire blight resistance in Malus × robusta 5 is coding for a CC-NBS-LRR [Elektronische Daten] [Johannes Fahrentrapp, Giovanni Broggini, Markus Kellerhals, Andreas Peil, Klaus Richter, Elena Zini, Cesare Gessler] Fire blight is the most important bacterial disease in apple (Malus ×  domestica) and pear (Pyrus communis) production. Today, the causal bacterium Erwinia amylovora is present in many apple- and pear-growing areas. We investigated the natural resistance of the wild apple Malus ×  robusta 5 against E. amylovora, previously mapped to linkage group 3. With a fine-mapping approach on a population of 2,133 individuals followed by phenotyping of the recombinants from the region of interest, we developed flanking markers useful for marker-assisted selection. Open reading frames were predicted on the sequence of a BAC spanning the resistance locus. One open reading frame coded for a protein belonging to the NBS-LRR family. The in silico investigation of the structure of the candidate resistance gene against fire blight of M. ×  robusta 5, FB_MR5, led us hypothesize the presence of a coiled-coil region followed by an NBS and an LRR-like structure with the consensus ‘LxxLx[IL]xxCxxLxxL'. The function of FB_MR5 was predicted in agreement with the decoy/guard model, that FB_MR5 monitors the transcribed RIN4_MR5, a homolog of RIN4 of Arabidopsis thaliana that could interact with the previously described effector AvrRpt2EA of E. amylovora. Springer-Verlag, 2012 Erwinia amylovora nationallicence Positional cloning nationallicence Fine mapping nationallicence QTL analysis nationallicence R gene nationallicence Apple breeding nationallicence Fahrentrapp Johannes Plant Pathology Integrative Biology Zurich (IBZ), Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland aut Broggini Giovanni Plant Pathology Integrative Biology Zurich (IBZ), Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland aut Kellerhals Markus Agroscope Changins-Wädenswil (ACW), Wädenswil, Switzerland aut Peil Andreas Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticulture and Fruit Crops, Dresden, Germany aut Richter Klaus Julius Kühn-Institut, Federal Research Center for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany aut Zini Elena Fondazione Edmund Mach-FEM, Istituto Agrario San Michele (IASMA), TN, S. Michele all'Adige, Italy aut Gessler Cesare Plant Pathology Integrative Biology Zurich (IBZ), Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland aut Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 237-251 1614-2942 9:1<237 2013 9 11295 https://doi.org/10.1007/s11295-012-0550-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11295-012-0550-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fahrentrapp Johannes Plant Pathology Integrative Biology Zurich (IBZ), Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland aut NATIONALLICENCE 700 1- Broggini Giovanni Plant Pathology Integrative Biology Zurich (IBZ), Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland aut NATIONALLICENCE 700 1- Kellerhals Markus Agroscope Changins-Wädenswil (ACW), Wädenswil, Switzerland aut NATIONALLICENCE 700 1- Peil Andreas Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticulture and Fruit Crops, Dresden, Germany aut NATIONALLICENCE 700 1- Richter Klaus Julius Kühn-Institut, Federal Research Center for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, Germany aut NATIONALLICENCE 700 1- Zini Elena Fondazione Edmund Mach-FEM, Istituto Agrario San Michele (IASMA), TN, S. Michele all'Adige, Italy aut NATIONALLICENCE 700 1- Gessler Cesare Plant Pathology Integrative Biology Zurich (IBZ), Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland aut NATIONALLICENCE 773 0- Tree Genetics & Genomes Springer-Verlag 9/1(2013-02-01), 237-251 1614-2942 9:1<237 2013 9 11295 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer